Tn3-like structures co-harboring of blaCTX-M-65, blaTEM-1 and blaOXA-10 in the plasmids of two Escherichia coli ST1508 strains originating from dairy cattle in China

文献类型： 外文期刊

第一作者： Wang, Weiwei

作者： Wang, Weiwei;Wei, Xiaojuan;Zhu, Zhen;Wu, Lingyu;Zhu, Qiqi;Arbab, Safia;Wang, Chengye;Bai, Yubin;Wang, Qing;Zhang, Jiyu;Wang, Weiwei;Wei, Xiaojuan;Zhu, Zhen;Wu, Lingyu;Zhu, Qiqi;Arbab, Safia;Wang, Chengye;Bai, Yubin;Wang, Qing;Zhang, Jiyu;Wang, Weiwei;Wei, Xiaojuan;Zhu, Zhen;Wu, Lingyu;Zhu, Qiqi;Arbab, Safia;Wang, Chengye;Bai, Yubin;Wang, Qing;Zhang, Jiyu;Zhu, Zhen;Zhu, Qiqi;Wang, Chengye;Wang, Qing

作者机构： Key Lab New Anim Drug Project Gansu Prov, Lanzhou 730050, Gansu, Peoples R China;Minist Agr, Key Lab Vet Pharmaceut Dev, Lanzhou 730050, Gansu, Peoples R China;Chinese Acad Agr Sci, Lanzhou Inst Husb & Pharmaceut Sci, Lanzhou 730050, Gansu, Peoples R China;Hebei Univ Engn, Coll Life Sci & Food Engn, Handan 056038, Hebei, Peoples R China;Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Gansu, Peoples R China

关键词： Bla(CTX-M-65); Tn3-like; Transmission; E. coli; Dairy cattle

期刊名称：BMC VETERINARY RESEARCH （ 2022影响因子：2.6； 五年影响因子：2.9 ）

ISSN：

年卷期： 2023 年 19 卷 1 期

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收录情况： SCI

摘要： The purpose of this study was to determine the level of horizontal transmission of the bla(CTX-M-65) gene and the role of its associated mobile genetic elements (MGEs) in the bovine-derived Escherichia coli. After PCR identification, two plasmids carrying bla(CTX-M-65) were successfully transferred to the recipient E. coli J53 Azr through conjugation assays and subsequently selected for Whole-Genome sequencing (WGS) analysis. The resistance profiles of these two positive strains and their transconjugants were also determined through antimicrobial susceptibility tests. Whole genome data were acquired using both the PacBio sequencing platform and the Illumina data platform. The annotated results were then submitted to the Genbank database for accession number recording. For comparison, the genetic environment of plasmids carrying the resistance gene bla(CTX-M-65) was mapped using the Easyfig software. WGS analysis revealed Tn3-like composite transposons bearing bla(CTX-M-65), bla(TEM-1), and bla(OXA-10) in the IncHI2-type plasmids of these two E. coli ST1508 strains. A phylogenetic tree was generated from all 48 assembled E. coli isolates bla(CTX-M-65), bla(TEM-1), and bla(OXA-10) from the NCBI Pathogen Detection database with our two isolates, showing the relationships and the contribution of SNPs to the diversity between genetic samples. This study suggests that the transmissibility of bla(CTX-M-65) on Tn3-like composite transposons contributes to an increased risk of its transmission in E. coli derived from dairy cattle.

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