Roles of estrogen receptors during sexual reversal in Pelodiscus sinensis

文献类型： 外文期刊

第一作者： Chen, Guobin

作者： Chen, Guobin;Zhou, Tong;Cao, Jizeng;Wang, Long;Zou, Guiwei;Liang, Hongwei;Cao, Jizeng;Li, Xiang;Zhu, Chengjun

作者机构：

关键词： Estrogen receptor; Sex differentiation; Pelodiscus sinensis; 17 beta-estradiol

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.8； 五年影响因子：2.7 ）

ISSN： 0301-4851

年卷期： 2024 年 51 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundThe Chinese soft-shelled turtle, Pelodiscus sinensis, exhibits distinct sexual dimorphism, with the males growing faster and larger than the females. During breeding, all-male offspring can be obtained using 17 beta-estradiol (E2). However, the molecular mechanisms underlying E2-induced sexual reversal have not yet been elucidated. Previous studies have investigated the molecular sequence and expression characteristics of estrogen receptors (ERs). Methods and ResultsIn this study, primary liver cells and embryos of P. sinensis were treated with ER agonists or inhibitors. Cell incubation experiments revealed that nuclear ERs (nERs) were the main pathway for the transmission of estrogen signals. Our results showed that ER alpha agonist (ER alpha-ag) upregulated the expression of Rspo1, whereas ER alpha inhibitor (ER alpha-Inh) downregulated its expression. The expression of Dmrt1 was enhanced after ER alpha-Inh + G-ag treatment, indicating that the regulation of male genes may not act through a single estrogen receptor, but a combination of ERs. In embryos, only the ER alpha-ag remarkably promoted the expression levels of Rspo1, Wnt4, and beta-catenin, whereas the ER alpha-Inh had a suppressive effect. Additionally, Dmrt1, Amh, and Sox9 expression levels were downregulated after ER beta inhibitor (ER beta-Inh) treatment. GPER agonist (G-ag) has a significant promotion effect on Rspo1, Wnt4, and beta-catenin, while the inhibitor G-Inh does not affect male-related genes. ConclusionsOverall, these results suggest that ERs play different roles during sexual reversal in P. sinensis and ER alpha may be the main carrier of estrogen-induced sexual reversal in P. sinensis. Further studies need to be performed to analyze the mechanism of ER action.

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