Analysis of flavonoids biosynthesis-related genes expression reveals the mechanism of difference of flavonoid content in different tissues of Actinidia arguta

文献类型： 外文期刊

第一作者： Tan, Changhua

作者： Tan, Changhua;Liu, Changjiang;Wang, Zhigang;Irfan, Muhammad

作者机构：

关键词： Correlation; Flavonoid composition; LC-MS; MS; Leaf

期刊名称：BRAZILIAN JOURNAL OF BOTANY （ 影响因子：1.296； 五年影响因子：1.394 ）

ISSN： 0100-8404

年卷期： 2021 年 44 卷 3 期

页码：

收录情况： SCI

摘要： Fruits of Actinidia arguta (Sieb. et Zucc.) Planch. ex Miq. are important source of bioactive substances including flavonoids. However, there were few reports on the phytochemical aspects of Actinidia arguta in different tissues. Here, we investigated the flavonoid content in different tissues of A. arguta by LC-MS/MS technology. In our study, ten flavonoids were detected, namely kaempferol-3-O-rutinoside (+), kaempferol-3-O-rutinoside (-), kaempferol-3-O-neohesperidoside (P), isorhamnetin-3-O-alpha-l-rhamnopyranosyl-(1 -> 3)-alpha-l-rhamnopyranosyl-(1 -> 6)-beta-d-galactopyranoside, isorhamnetin-3-O-alpha-l-rhamnopyranosyl-(1 -> 3)-alpha-l-rhamnopyranosyl-(1 -> 6)-beta-d-galactopyranoside, isorhamnetin-3-O-neohesperidoside (P)(+), isorhamnetin-3-O-neohesperidoside (P)(-), isorhamnetin-3-O-rutinoside, isorhamnetin-3-O-neohespeidoside, quercetin -3-O- rhamnoglycoside, which were belonged to kaempferol, isorhamnetin and quercetin, the major flavonoid compounds in Actinidia arguta, which decreased with the order of leaf > petiole > fruit. In young apical leaves, the total flavonoid content was 6.3-fold higher than that in fruit. And there was a continuous decrease as leaf development in total flavonoids content. In addition, except AaCHS-2 and AaDFR, the expression levels of nine genes-related flavonoid biosynthesis were similar to the change of total flavonoids content in different tissues. It indicated that the eleven genes played main regulation in flavonoid biosynthesis. Thus, our results revealed the regulatory information of flavonoid biosynthesis in Actinidia arguta leaves and provide technical support for the full utilization of leaves.

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