Effects of Low Nighttime Temperature on Fatty Acid Content in Developing Seeds from Brassica napus L. Based on RNA-Seq and Metabolome

文献类型： 外文期刊

第一作者： Mi, Chao

作者： Mi, Chao;Sun, Chao;Lin, Liangbin;Yuan, Yuting;Li, Fei;Wang, Qian;Zhu, Haiping;Hua, Shuijin

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关键词： Brassica napus L; seeds; nighttime temperature; fatty acid content; RNA-seq; metabolome; cis-acting element involved in low-temperature responsiveness; oleic acid; erucic acid; HB1; KCS

期刊名称：PLANTS-BASEL （ 影响因子：4.5； 五年影响因子：4.8 ）

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年卷期： 2023 年 12 卷 2 期

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收录情况： SCI

摘要： Brassica napus L. is a vital plant oil resource worldwide. The fatty acid biosynthesis and oil accumulation in its seeds are controlled by several genetic and environmental factors, including daytime and nighttime temperatures. We analyzed changes in oleic and erucic acid content in two double haploid (DH) lines, DH0729, a weakly temperature-sensitive line, and DH0815, a strongly temperature-sensitive line, derived from B. napus plants grown at different altitudes (1600, 1800, 2000, 2200, and 2400 m a.s.l., 28.85 degrees N, 112.35 degrees E) and nighttime temperatures (20/18, 20/16, 20/13 and 20/10 degrees C, daytime/nighttime temperature). Based on medium- and long-chain fatty acid metabolites, the total oleic acid content 35 and 43 days after flowering was significantly lower in low nighttime temperature (LNT, 20/13 degrees C) plants than in high nighttime temperature (HNT, 20/18 degrees C) plants (HNT: 58-62%; LNT: 49-54%; an average decrease of 9%), and the total erucic acid content was significantly lower in HNT than in LNT plants (HNT: 1-2%; LNT: 8-13%; an average increase of 10%). An RNA-seq analysis showed that the expression levels of SAD (LOC106366808), ECR (LOC106396280), KCS (LOC106419344), KAR (LOC106367337), HB1(LOC106430193), and DOF5 (LOC111211868) in STSL seeds increased under LNT conditions. In STSL seeds, a base mutation in the cis-acting element involved in low-temperature responsiveness (LTR), the HB1 and KCS promoter caused loss of sensitivity to low temperatures, whereas that of the KCS promoter caused increased sensitivity to low temperatures.

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