Testing the polar auxin transport model with a selective plasma membrane H+-ATPase inhibitor

文献类型： 外文期刊

第一作者： Yang, Yongqing

作者： Yang, Yongqing;Zhao, Jun;Li, Junhong;Dong, Qing;Ma, Liang;Li, Yingzhang;Liu, Xiaohui;Lei, Xiaoguang;Liu, Xiaohui;Lei, Xiaoguang;Guo, Wei;Liu, Wei;Shao, Wei;He, Qun;Han, Jianyong

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关键词： Arabidopsis; auxin transport; inhibitor; plasma membrane H+-ATPase

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：9.106； 五年影响因子：8.241 ）

ISSN： 1672-9072

年卷期： 2022 年 64 卷 6 期

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收录情况： SCI

摘要： Auxin is unique among plant hormones in that its function requires polarized transport across plant cells. A chemiosmotic model was proposed to explain how polar auxin transport is derived by the H+ gradient across the plasma membrane (PM) established by PM H+-adenosine triphosphatases (ATPases). However, a classical genetic approach by mutations in PM H+-ATPase members did not result in the ablation of polar auxin distribution, possibly due to functional redundancy in this gene family. To confirm the crucial role of PM H+-ATPases in the polar auxin transport model, we employed a chemical genetic approach. Through a chemical screen, we identified protonstatin-1 (PS-1), a selective small-molecule inhibitor of PM H+-ATPase activity that inhibits auxin transport. Assays with transgenic plants and yeast strains showed that the activity of PM H+-ATPases affects auxin uptake as well as acropetal and basipetal polar auxin transport. We propose that PS-1 can be used as a tool to interrogate the function of PM H+-ATPases. Our results support the chemiosmotic model in which PM H+-ATPase itself plays a fundamental role in polar auxin transport.

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