A C2H2-type zinc finger protein TaZFP8-5B negatively regulates disease resistance

文献类型： 外文期刊

第一作者： Huang, Lin

作者： Huang, Lin;Huang, Yanyan;Xu, Qiang;Feng, Lihua;Zhang, Lianquan;Huang, Lin;Xie, Ruijie;Hu, Yanling;Du, Lilin;Wang, Fang;Zhao, Xueer;Chen, Xuejiao;Hao, Ming;Wu, Bihua;Liu, Dengcai;Wei, Zhenzhen

作者机构：

关键词： Wheat; Cys2/His2-type transcription factor; Calmodulin-like protein; Plant immunity

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.8； 五年影响因子：5.4 ）

ISSN： 1471-2229

年卷期： 2024 年 24 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundZinc finger proteins (ZFPs) are important regulators in abiotic and biotic stress tolerance in plants. However, the role of the ZFPs in wheat responding to pathogen infection is poorly understood.ResultsIn this study, we found TaZFP8-5B was down-regulated by Puccinia striiformis f. sp. tritici (Pst) infection. TaZFP8-5B possesses a single C2H2-type zinc finger domain with a plant-specific QALGGH motif, and an EAR motif (LxLxL) at the C-terminus. The EAR motif represses the trans-activation ability of TaZFP8-5B. Knocking down the expression of TaZFP8 by virus-induced gene silencing increased wheat resistance to Pst, whereas TaZFP8-5B-overexpressing reduced wheat resistance to stripe rust and rice resistance to Magnaporthe oryzae, suggesting that TaZFP8-5B plays a negative role in the modulation of plant immunity. Using bimolecular fluorescence complementation, split-luciferase, and yeast two-hybrid assays, we showed that TaZFP8-5B interacted with a wheat calmodulin-like protein TaCML21. Knock-down of TaCML21 reduced wheat resistance to Pst.ConclusionsThis study characterized the function of TaZFP8-5B and its interacting protein TaCML21. Our findings provide a new perspective on a regulatory module made up of TaCML21-TaZFP8-5B in plant immunity.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序