Phospholipase C-?1 potentially facilitates subcellular localization of activated ?-catenin, p-?-catenin(S552), during bovine herpesvirus 1 productive infection in MDBK cells

文献类型： 外文期刊

第一作者： Liu, Chang

作者： Liu, Chang;Ding, Xiuyan;Zhu, Liqian;Chang, Long;Yuan, Weifeng;Li, Shitao;Zhu, Liqian

作者机构：

关键词： Bovine herpesvirus 1; ?-catenin; Phospholipase C; ?1

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.246； 五年影响因子：3.565 ）

ISSN： 0378-1135

年卷期： 2023 年 276 卷

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收录情况： SCI

摘要： Bovine herpesvirus 1 (BoHV-1) is a significant risk factor for the bovine respiratory disease complex (BRDC), a severe disease causing great economic losses to the cattle industry worldwide. Previous studies have reported that both phospholipase C-gamma 1 (PLC-gamma 1) and beta-catenin are activated during BoHV-1 infection for efficient replication. However, the interplay between PLC-gamma 1 and beta-catenin as a consequence of virus infection remains to be elucidated. Here, we reported that PLC-gamma 1 interacted with beta-catenin, which was enhanced following virus infection. PLC-gamma 1-specific inhibitor, U73122, significantly reduced the mRNA levels of beta-catenin in BoHV-1infected cells; however, the steady-state protein levels were not affected due to the virus infection. Interestingly, the treatment of virus-infected cells with U73122 reduced the accumulation of activated beta-catenin [p beta-catenin(S552)] in fractions of the cytoplasmic membrane as that observed with the treatment of methyl beta-cyclodextrin (M beta CD), which can disrupt cytoplasmic membrane structure via sequestering cholesterol. Nucleus accumulation of p-beta-catenin(S552) was increased following U73122 treatment in virus-infected cells. In addition, the association of p-beta-catenin(S552) with cytoplasmic membrane induced by the virus infection was significantly disrupted by the treatment of U73122 and M beta CD. These data indicated that the PLC-gamma 1 signaling is potentially involved in the regulation of beta-catenin signaling stimulated by BoHV-1 infection partially via affecting the subcellular localization of p-beta-catenin(S552).

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