The cotton miR171a-SCL6 module mediates plant resistance through regulating GhPR1 expression

文献类型： 外文期刊

第一作者： Hu, Guang

作者： Hu, Guang;Ge, Xiaoyang;Wang, Peng;Li, Fuguang;Hu, Guang;Wu, Jiahe;Chen, Aimin

作者机构：

关键词： miR171a; SCL6; Verticillium wilt; Plant resistance; Gossypium hirsutum

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：6.5； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2023 年 202 卷

页码：

收录情况： SCI

摘要： Plants have developed intricate defense mechanisms in response to fluctuating environmental cues, including the use of microRNA (miRNA) as post-transcriptional regulators. However, the specific mechanisms through which miRNA contributes to disease resistance remain largely elusive. While the miR171-SCLs have been investigated in an eclectic array of plants, there has been a notable scarcity of research specifically focused on cotton (Gossypium hirsutum). In our previous miRNA-sequencing analysis, we found that ghr-miR171a displayed a differential response to infections by Verticillium dahliae. In this study, we further investigated the function of the miR171a-SCL6 module in cotton during V. dahliae infection. The ghr-miR171a was confirmed to direct the cleavage of GhSCL6 mRNA in the post-transcriptional process, as evidenced by 5 ' RLM-RACE, beta-glucuronidase (GUS) histochemical staining and enzyme activity assay. Interestingly, we found that overexpressing ghrmiR171a reduced cotton plants' resistance to V. dahliae, while suppressing ghr-miR171a increased the plants' defense capacity. The GhSCL6 protein, when fused with green fluorescent protein (GFP), localizes in the cell nucleus, indicating its potential role in gene regulation. This was further corroborated by yeast two-hybrid assays, which verified GhSCL6's transcriptional activation ability. Through quantitative reverse transcriptase PCR (qRT-PCR), luciferase (LUC) fluorescence, and yeast one-hybrid assays, we found that GhSCL6 binds to the GTbox element of the GhPR1 promoter, activating its expression and thereby enhancing plant disease resistance. Taken together, our findings demonstrate that the cotton miR171a-SCL6 module regulates Verticillium wilt resistance in plants through the post-transcriptional process. This insight may offer new perspectives for disease resistance strategies in cotton.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序