The role of Nrf2 signaling pathway in the mud crab (Scylla paramamosain) in response to Vibrio parahaemolyticus infection

文献类型： 外文期刊

第一作者： Cheng, ChangHong

作者： Cheng, ChangHong;Ma, HongLing;Liu, GuangXin;Fan, SiGang;Deng, YiQin;Jiang, JianJun;Feng, Juan;Guo, ZhiXun;Guo, ZhiXun

作者机构：

关键词： Mud crab; Nrf2; Immune response; Bacterial challenge

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 1050-4648

年卷期： 2023 年 136 卷

页码：

收录情况： SCI

摘要： The transcription factor Nrf2 plays vital roles in detoxification and antioxidant enzymes against oxidative stress. However, the function of Nrf2 in crustaceans is not well studied. In this study, a novel Nrf2 gene from the mud crab (Sp-Nrf2) was identified. It was encoded 245 amino acids. Sp-Nrf2 expression was ubiquitously expressed in all tested tissues, with the highest expression level in the gill. Sp-Nrf2 protein was mainly located in the nucleus. The expression levels of Sp-Nrf2, and antioxidant-related genes (HO-1 and NQO-1) were induced after Vibrio parahaemolyticus infection, indicating that Nrf2 signaling pathway was involved in the responses to bacterial infection. Over-expression of Sp-Nrf2 could improve cell viability after H2O2 exposure, indicating that Sp-Nrf2 might relieve oxidative stress. Silencing of Sp-Nrf2 in vivo decreased HO-1 and NQO-1 expression. Moreover, knocking down Sp-Nrf2 in vivo can increase malondialdehyde content and the mortality of mud crabs after V. parahaemolyticus infection. Our results indicated that Nrf2 signaling pathway played a significant role in immune response against bacterial infection.

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