Metal-Enhanced Near Infrared Fluorescence-Based Sensor with Highly Improved Sensitivity for Adenosine Triphosphate

文献类型： 外文期刊

第一作者： Yu, Manli

作者： Yu, Manli;Cui, Bo;Sun, Changjiao;Zhao, Xiang;Wang, Yan;Liu, Guoqiang;Cui, Haixin;Zeng, Zhanghua;Yao, Yishan

作者机构：

关键词： near-infrared fluorescence; metal-enhanced fluorescence; ATP; gold nanorods; aptamers

期刊名称：ACS APPLIED NANO MATERIALS （ 影响因子：5.097； 五年影响因子：5.1 ）

ISSN： 2574-0970

年卷期： 2019 年 2 卷 1 期

页码：

收录情况： SCI

摘要： Near infrared (NIR) fluorescent sensor for biologically important analyte is very promising because NIR fluorescence imaging has minimal autofluorescence and high tissue penetration in living system. Nevertheless, its low fluorescence quantum yield leads to low sensitivity of detection. In this study, a functionalized gold nanorods (NIR-AuNRs-3) plasmon enhanced NIR fluorescence-based sensor for adenosine triphosphate (ATP) was developed with highly improved sensitivity. The fluorescence quantum yield with ATP was significantly enhanced, from almost zero to 0.61, resulting in remarkable enhancement of sensitivity. This was attributed to the increase of radiative decay rate of NIR dye by 30-fold, along with almost unchanged nonradiative decay rate. The limit of detection for ATP was 0.6 nM, and it was successfully applied to determine low ATP levels (10.1 +/- 2.6 nM) in rat brain. It has been also demonstrated to be useful for monitoring intracellular ATP levels with high sensitivity.

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