Analysis of microbial utilization of rice straw in paddy soil using a DNA-SIP approach

文献类型： 外文期刊

第一作者： Guo, Tengfei

作者： Guo, Tengfei;Ai, Chao;Liang, Guoqing;He, Ping;Lei, Qiuliang;Zhou, Wei;Zhang, Qian

作者机构：

期刊名称：SOIL SCIENCE SOCIETY OF AMERICA JOURNAL （ 影响因子：2.307； 五年影响因子：2.832 ）

ISSN： 0361-5995

年卷期： 2020 年 84 卷 1 期

页码：

收录情况： SCI

摘要： Considering the close connection between soil microorganisms with carbon (C) cycling, the aim of this study was to identify the specific bacterial and fungal microbes that assimilate C-13 from incorporated rice straw and explore their dynamics and characteristics during straw decomposition in paddy soil. Soil microcosms based on C-12/13-labeled rice straw were incubated with the determination of CO2 production at 1, 3, 7, 14, 28, 56 and 90 days after straw incorporation. Meanwhile, the targeted soil bacterial and fungal communities were characterized using a DNA-based stable isotope probing (DNA-SIP) approach combined with Illumina MiSeq sequencing. Rice straw decomposed rapidly in the first 2 weeks, coupled with a large turnover of soil native organic matter. During this process, Actinobacteria including the orders Streptomycetales, Caternulisporales and Corynebacteriales dominated the community utilizing rice straw-derived C with a succession from Streptomyces, to Kitasatospora, to Catenulispora. At Days 56 to 90, the dominant orders assimilating rice straw-derived C-13 were Micrococcales, Sphingobacteriia, Gammaproteobacteria from phyla Actinobacteria, Bacteroidetes and Proteobacteria, respectively. The fungal orders Onygenales, Capnodiales, Sordariales and Pleosporales showed stronger ability of C-13 utilization at late decomposition stage. Taken together, along with stimulation of soil organic matter mineralization after rice straw addition, dynamics of C-13-assimilating bacterial and fungal groups with various characteristics were identified.

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