The high-affinity phosphodiesterase PcPdeH is involved in the polarized growth and pathogenicity of Phytophthora capsici

文献类型： 外文期刊

第一作者： Li, X.

作者： Li, X.;Liu, Yong;Tan, Xinqiu;Zhang, Deyong;Li, X.;Liu, Yong;Tan, Xinqiu;Zhang, Xin;Zhang, Deyong;Li, Delong;Yang, Xinyu

作者机构：

关键词： cAMP phosphodiesterases; Cyst germination; Oomycete; Virulence

期刊名称：FUNGAL BIOLOGY （ 影响因子：3.099； 五年影响因子：3.364 ）

ISSN： 1878-6146

年卷期： 2020 年 124 卷 3-4 期

页码：

收录情况： SCI

摘要： The cAMP signaling pathway has been shown to be important in controlling morphological changes and pathogenicity in plant pathogens. In the present study, we identified PcPdeH, a gene encoding a high-affinity phosphodiesterase (PDE), which is a key regulator of the cAMP signaling pathway. To elucidate the function of PcPdeH, PcPdeH-knockout mutants were obtained using a type II CRISPR/Cas9 system in Phytophthora capsici. The knockout transformants of PcPdeH showed vegetative growth defects and abnormal cyst germination. Infection assays indicated that compared with the wild type, PcPdeH-knockout mutants showed significantly reduced virulence on pepper and tobacco leaves and exhibited increased (1.5-2-fold) cAMP levels relative to the wild-type and CK strains. Based on these phenotypic features, we propose that PcPdeH is crucial for vegetative growth, cyst germination and pathogenicity in P. capsici. (C) 2020 The Author(s). Published by Elsevier Ltd on behalf of British Mycological Society.

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