An integrated multiple reaction monitoring strategy based on predicted precursor ions and characteristic product ions for global profiling Rubiaceae-type cyclopeptides in three Rubia species

文献类型： 外文期刊

第一作者： Bi, Qirui

作者： Bi, Qirui;Liu, Qiao;Han, Sha;Zhang, Lu;Zhang, Xuejia;Wang, Zhe;Miao, Yuanyuan;Tan, Ninghua;Xing, Longsheng

作者机构：

关键词： Rubiaceae-type cyclopeptides; Predicted precursor ions; Characteristic product ions; Rubia; Species discrimination; Chemical markers

期刊名称：JOURNAL OF CHROMATOGRAPHY A （ 影响因子：4.759； 五年影响因子：4.302 ）

ISSN： 0021-9673

年卷期： 2020 年 1618 卷

页码：

收录情况： SCI

摘要： Complexity and diversity of natural compounds make it a challenge for globally profiling constituents in multiple species plants, especially for minor new compounds. Rubiaceae-type cyclopeptides (RAs) are one kind of active constituents and characteristic components in Rubia plants, particularly Rubia cordifolia (RC), which is one kind of traditional Chinese medicines. In this study, a new multiple reaction monitoring strategy (PPCP-MRM) based on predicted precursor ions and characteristic product ions was developed to globally profile RAs in RC and its two main adulterants, including Rubia alata (also named Jinjiancao in Chinese) (RAJ) and Rubia podantha (RP). Moreover, characteristic components of these species have been found by targeted relative quantitative analysis of RAs by LC-MS. In total, 39 RAs have been structurally annotated based on fragment ions in MS2 data, including 19 new compounds. In addition, 7 RAs as the chemical markers were found to distinguish these three Rubia species. The results indicated that this PPCP-MRM integrated strategy is a powerful tool for comprehensive analysis of RAs and screening chemical markers for Rubia species discrimination, which would be useful for distinguishing Rubia adulterants. Furthermore, this developed strategy could also be a useful tool for analysis of other cyclopeptides. (C) 2020 Elsevier B.V. All rights reserved.

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