Oocyte IVM or vitrification significantly impairs DNA methylation patterns in blastocysts as analysed by single-cell whole-genome methylation sequencing

文献类型： 外文期刊

第一作者： Zhao, Ya-Han

作者： Zhao, Ya-Han;Wang, Jing-Jing;Zhang, Pei-Pei;Hao, Hai-Sheng;Pang, Yun-Wei;Wang, Hao-Yu;Du, Wei-Hua;Zhao, Shan-Jiang;Zou, Hui-Ying;Hao, Tong;Zhu, Hua-Bin;Zhao, Xue-Ming;Zhao, Ya-Han;Wang, Jing-Jing;Zhang, Pei-Pei;Hao, Hai-Sheng;Pang, Yun-Wei;Wang, Hao-Yu;Du, Wei-Hua;Zhao, Shan-Jiang;Zou, Hui-Ying;Hao, Tong;Zhu, Hua-Bin;Zhao, Xue-Ming;Ruan, Wei-Min

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关键词： bovine; development; embryo; mechanism

期刊名称：REPRODUCTION FERTILITY AND DEVELOPMENT （ 影响因子：2.311； 五年影响因子：2.396 ）

ISSN： 1031-3613

年卷期： 2020 年 32 卷 7 期

页码：

收录情况： SCI

摘要： To explore the mechanisms leading to the poor quality of IVF blastocysts, the single-cell whole-genome methylation sequencing technique was used in this study to analyse the methylation patterns of bovine blastocysts derived from in vivo, fresh (IVF) or vitrified (V_IVF) oocytes. Genome methylation levels of blastocysts in the IVF and V_IVF groups were significantly lower than those of the in vivo group (P < 0.05). In all, 1149 differentially methylated regions (DMRs) were identified between the IVF and in vivo groups, 1578 DMRs were identified between the V_IVF and in vivo groups and 151 DMRs were identified between the V_IVF and IVF groups. For imprinted genes, methylation levels of insulin-like growth factor 2 receptor (IGF2R) and protein phosphatase 1 regulatory subunit 9A (PPP1R9A) were lower in the IVF and V_IVF groups than in the in vivo group, and the methylation level of paternally expressed 3 (PEG3) was lower in the V_IVF group than in the IVF and in vivo groups. Genes with DMRs between the IVF and in vivo and the V_IVF and IVF groups were primarily enriched in oocyte maturation pathways, whereas DMR sbetween the V_IVF and in vivo groups were enriched in fertilisation and vitrification-vulnerable pathways. The results of this study indicate that differences in the methylation of critical DMRs may contribute to the differences in quality between in vitro- and in vivo-derived embryos.

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