GWAS and co-expression network combination uncovers multigenes with close linkage effects on the oleic acid content accumulation in Brassica napus

文献类型： 外文期刊

第一作者： Yao, Min

作者： Yao, Min;Guan, Mei;Zhang, Zhenqian;Zhang, Qiuping;Chen, Hao;Liu, Wei;He, Xin;Liu, Zhongsong;Guan, Chunyun;Hua, Wei;Qian, Lunwen;Cui, Yixin;Jan, Habib U.;Voss-Fels, Kai P.;Werner, Christian R.;Snowdon, Rod J.;Hua, Wei

作者机构：

关键词： Oleic acid; GWAS; Haplotype; Co-expression network; Brassica napus

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2020 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Background Strong artificial and natural selection causes the formation of highly conserved haplotypes that harbor agronomically important genes. GWAS combination with haplotype analysis has evolved as an effective method to dissect the genetic architecture of complex traits in crop species. Results We used the 60 K Brassica Infinium SNP array to perform a genome-wide analysis of haplotype blocks associated with oleic acid (C18:1) in rapeseed. Six haplotype regions were identified as significantly associated with oleic acid (C18:1) that mapped to chromosomes A02, A07, A08, C01, C02, and C03. Additionally, whole-genome sequencing of 50 rapeseed accessions revealed three genes (BnmtACP2-A02, BnABCI13-A02 and BnECI1-A02) in the A02 chromosome haplotype region and two genes (BnFAD8-C02 and BnSDP1-C02) in the C02 chromosome haplotype region that were closely linked to oleic acid content phenotypic variation. Moreover, the co-expression network analysis uncovered candidate genes from these two different haplotype regions with potential regulatory interrelationships with oleic acid content accumulation. Conclusions Our results suggest that several candidate genes are closely linked, which provides us with an opportunity to develop functional haplotype markers for the improvement of the oleic acid content in rapeseed.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序