Pyrophosphate-fructose 6-phosphate 1-phosphotransferase (PFP1) regulates starch biosynthesis and seed development via heterotetramer formation in rice (Oryza sativa L.)

文献类型： 外文期刊

第一作者： Chen, Chen

作者： Chen, Chen;Zhang, Peng;Yin, Junliang;Fang, Zhengwu;Chen, Chen;He, Bingshu;Zhang, Peng;Wei, Xin;Qiao, Yongli;He, Bingshu;Liu, Xingxun;Ma, Xiaoding;Liu, Yujie;Yang, Chen;Yao, Hong-Yan;Xue, Hong-Wei;Yao, Hong-Yan;Xue, Hong-Wei;Koh, Hee-Jong;Koh, Hee-Jong

作者机构：

关键词： floury endosperm; rice; pyrophosphate-fructose 6-phosphate 1-phosphotransferase (PFP1); map-based cloning; starch synthesis

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：9.803； 五年影响因子：9.555 ）

ISSN： 1467-7644

年卷期： 2020 年 18 卷 1 期

页码：

收录情况： SCI

摘要： Pyrophosphate-fructose 6-phosphate 1-phosphotransferase (PFP1) reversibly converts fructose 6-phosphate and pyrophosphate to fructose 1, 6-bisphosphate and orthophosphate during glycolysis, and has diverse functions in plants. However, mechanisms underlying the regulation of starch metabolism by PFP1 remain elusive. This study addressed the function of PFP1 in rice floury endosperm and defective grain filling. Compared with the wild type, pfp1-3 exhibited remarkably low grain weight and starch content, significantly increased protein and lipid content, and altered starch physicochemical properties and changes in embryo development. Map-based cloning revealed that pfp1-3 is a novel allele and encodes the regulatory beta-subunit of PFP1 (PFP1 beta). Measurement of nicotinamide adenine dinucleotide (NAD+) showed that mutation of PFP1 beta markedly decreased its enzyme activity. PFP1 beta and three of four putative catalytic alpha-subunits of PFP1, PFP1 alpha 1, PFP1 alpha 2, and PFP1 alpha 4, interacted with each other to form a heterotetramer. Additionally, PFP1 beta, PFP1 alpha 1 and PFP1 alpha 2 also formed homodimers. Furthermore, transcriptome analysis revealed that mutation of PFP1 beta significantly altered expression of many essential enzymes in starch biosynthesis pathways. Concentrations of multiple lipid and glycolytic intermediates and trehalose metabolites were elevated in pfp1-3 endosperm, indicating that PFP1 modulates endosperm metabolism, potentially through reversible adjustments to metabolic fluxes. Taken together, these findings provide new insights into seed endosperm development and starch biosynthesis and will help in the breeding of rice cultivars with higher grain yield and quality.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序