Candidate genes association study to identify allele-specific SNP marker of omega-3 fatty acid in Brassica napus

文献类型： 外文期刊

第一作者： Zafar, Sundus

作者： Zafar, Sundus;Zafar, Sundus;Tan, Xiao-Li;Tang, Min-Qiang;Liu, Sheng-Yi

作者机构： Jiangsu Univ, Sch Agr Equipment Engn, Zhenjiang 212013, Jiangsu, Peoples R China;Jiangsu Univ, Inst Life Sci, Zhenjiang 212013, Jiangsu, Peoples R China;Chinese Acad Agr Sci CAAS, Oil Crops Res Inst OCRI, Wuhan, Hubei, Peoples R China

关键词： Brassica napus; omega-3 fatty acid; Saccharomyces cerevisiae; Single nuceotide polymorphism

期刊名称：JOURNAL OF PLANT PHYSIOLOGY （ 2020影响因子：3.549； 五年影响因子：4.164 ）

ISSN： 0176-1617

年卷期： 2020 年 248 卷

页码：

收录情况： SCI

摘要： Due to the rapid decline in oceanic fish stock, omega-3 fatty acid (C18:3) has attracted serious attention and, hence, the identification of genotypes with high omega-3 content has become the main objective of Brassica napus (rapeseed) breeding. A candidate genes association study permitted us to delineate a genomic region linked to omega-3 content, offering a detailed understanding of the complex genetic mechanism of fatty acid biosynthesis in B. napus. Herein, the candidate genes association study, conducted on 324 genetically diverse rapeseed accessions, detected 114 single nucleotide polymorphisms (SNPs) associated with omega-3 fatty acid. Furthermore, these loci were functionally characterized in Saccharomyces cerevisiae. These associated loci were selected based on their contribution to a high C18:3 ratio, and the selected candidate loci were validated using allele-specific SNPs markers in an inbred population through polymerase chain reaction (PCR). These findings may contribute to improving the fatty acid composition by marker-based breeding and will facilitate the development of rapeseed varieties with high omega-3 content.

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