文献类型： 外文期刊
第一作者： Zheng, Xuefang
作者： Zheng, Xuefang;Wang, Jieping;Zhang, Haifeng;Liu, Bo;Wang, Ziran
作者机构：
期刊名称：CURRENT MICROBIOLOGY （ 影响因子：2.188； 五年影响因子：2.197 ）
ISSN： 0343-8651
年卷期： 2020 年 77 卷 9 期
页码：
收录情况： SCI
摘要： A Gram-staining-positive, motile, rod-shaped, aerobic bacterial strain FJAT-25496( T) was isolated from a forest soil sample collected from Dafu County, Chengdu City, Sichuan Province, China. Strain FJAT-25496( T) grew at 15-50 degrees C (optimum 35 degrees C), pH 6.0-10.0 (optimum 8.0), and NaCl tolerance of 0-4.0% (w/v) (optimum 0%). The strain contained iso-C-15:0 and anteiso-C-15:0 as the predominant cellular fatty acids as well as MK-7 as the only menaquinone. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), and phosphatidyl glycerol (PG). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain FJAT-25496( T) was a member of the genus Bacillus, and most closely related to Bacillus solani FJAT-18043( T) (97.8%), Bacillus praedii FJAT-25547( T) (97.8%), Bacillus depressus BZ1(T) (97.7%), Bacillus oceanisediminis H2(T) (97.7%), and Bacillus ciccensis 5L6(T) (97.6%). Based on complete genome comparisons between strain FJAT-25496( T) and the type strains of its closely related species, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values were 73.4-76.3% and 21.7-23.2%, respectively. The genomic DNA G + C content of strain FJAT-25496( T) was 36.9%. On the basis of phenotypic, phylogenetic, chemotaxonomic, and genomic features, strain FJAT-25496( T) is considered to represent a novel species of the genus Bacillus, for which the name Bacillus dafuensis sp. nov. is proposed. The type strain is FJAT-25496( T) (= CCTCC AB 2019182( T) = KCTC 43120( T)).
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