V3 protein encoded by mulberry crinkle leaf virus acts as a pathogenicity determinant in Nicotiana benthamiana
文献类型： 外文期刊
第一作者： Lu, Quan-You
作者： Lu, Quan-You;Yu, Jing;Cheng, Yong-Yuan;Sun, Xin;Yang, Lei;Lu, Quan-You
作者机构：
关键词： Mulberry crinkle leaf virus; Geminivirus; Pathogenicity determinant; Essential amino acid sequence
期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）
ISSN： 0929-1873
年卷期： 2020 年 157 卷 1 期
页码：
收录情况： SCI
摘要： A potential pathogenicity determinant and its critical amino acid (aa) sequences of mulberry crinkle leaf virus (MCLV) were determined through expression of MCLV genes in Nicotiana benthamiana using a potato virus X (PVX) vector. Among six genes encoded by MCLV, expression of only V3 resulted in a severe downward-leaf-curling symptom in newly emerging leaves of N. benthamiana plants, which suggested that the V3 protein is a pathogenicity determinant of MCLV. Analyses of truncation mutations indicated that the 94 residues at the N terminus of V3 are sufficient to maintain the function of the pathogenicity determinant V3 in N. benthamiana.
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