Characterization of a Novel Interleukin-1 Receptor Antagonist from Sheep (Ovis aries)

文献类型： 外文期刊

第一作者： Zhang, Shi-Jun

作者： Zhang, Shi-Jun;Guo, Xing;Hu, Pan;Lu, Shi-Ying;Liu, Nan-Nan;Li, Yan-Song;Wang, Lu-Lu;Chang, Jiang;Chang, Heng-Zhen;Liu, Zeng-Shan;Zhou, Yu;Ren, Hong-Lin;Guo, Xing;Fu, Bao-Quan;Wang, Nan

作者机构：

关键词： Interleukin-1 receptor antagonist; Ovis aries; molecular characteristics; bioactivity; monoclonal antibody; gene expression; bacterial infection

期刊名称：JOURNAL OF INTERFERON AND CYTOKINE RESEARCH （ 影响因子：2.607； 五年影响因子：2.941 ）

ISSN： 1079-9907

年卷期： 2020 年 40 卷 5 期

页码：

收录情况： SCI

摘要： Interleukin-1 receptor antagonist (IL-1Ra) is an antagonist of IL-1 beta binding IL-1 beta receptors but does not induce intracellular responses or signal transduction. In this study, the full-length complementary DNA (cDNA) of the IL-1Ra gene (OaIL-1Ra) was identified from sheep (Ovis aries) using rapid amplification of cDNA ends PCR and submitted to GenBank with the accession number KC425613. The OaIL-1Ra cDNA comprised an open reading frame of 525 bp encoding a protein of 19765.8 Da, a 5 '-untranslated region (UTR) of 27 bp, and a 3 '-UTR of 676 bp with a poly(A) tail. Recombinant OaIL-1Ra with bioactivity was expressed in a prokaryotic expression system, and a monoclonal antibody against native OaIL-1Ra was prepared. Through Western blot analyses, the OaIL-1Ra protein was widely expressed in lung, heart, spleen, liver, kidney, muscle, intestine, lymphonodi, rumen, and white blood cells, with the highest levels in liver and spleen. The expression of OaIL-1Ra in primary cultured white blood cells of sheep were highly induced in a time-dependent manner when challenged with different bacteria. These results implied that OaIL-1Ra is associated with immune responses during bacterial infections.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序