Genome-wide analysis of mRNA and lncRNA expression and mitochondrial genome sequencing provide insights into the mechanisms underlying a novel cytoplasmic male sterility system, BVRC-CMS96, in Brassicarapa

文献类型： 外文期刊

第一作者： Li, Peirong

作者： Li, Peirong;Zhang, Deshuang;Su, Tongbing;Wang, Weihong;Yu, Yangjun;Zhao, Xiuyun;Li, Zhenxing;Yu, Shuancang;Zhang, Fenglan;Li, Peirong;Zhang, Deshuang;Su, Tongbing;Wang, Weihong;Yu, Yangjun;Zhao, Xiuyun;Li, Zhenxing;Yu, Shuancang;Zhang, Fenglan;Li, Peirong;Zhang, Deshuang;Su, Tongbing;Wang, Weihong;Yu, Yangjun;Zhao, Xiuyun;Li, Zhenxing;Yu, Shuancang;Zhang, Fenglan

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关键词： Cytoplasmic male sterility; Mitochondrial genome; Chinese cabbage; BVRC-CMS96; orf138; orf222

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）

ISSN： 0040-5752

年卷期： 2020 年 133 卷 7 期

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收录情况： SCI

摘要： Key message Characterization of a novel and valuable CMS system in Brassicarapa. Cytoplasmic male sterility (CMS) is extensively used to produce F-1 hybrid seeds in a variety of crops. However, it has not been successfully used in Chinese cabbage (Brassicarapa L. ssp. pekinensis) because of degeneration or temperature sensitivity. Here, we characterize a novel CMS system, BVRC-CMS96, which originated in B.napus cybrid obtained from INRAE, France and transferred by us to B.rapa. Floral morphology and agronomic characteristics indicate that BVRC-CMS96 plants are 100% male sterile and show no degeneration in the BC7 generation, confirming its suitability for commercial use. We also sequenced the BVRC-CMS96 and maintainer line 18BCM mitochondrial genomes. Genomic analyses showed the presence of syntenic blocks and distinct structures between BVRC-CMS96 and 18BCM and the other known CMS systems. We found that BVRC-CMS96 has one orf222 from 'Nap'-type CMS and two copies of orf138 from 'Ogu'-type CMS. We analyzed expression of orf222, orf138, orf261b, and the mitochondrial energy genes (atp6, atp9, and cox1) in flower bud developmental stages S1-S5 and in four floral organs. orf138 and orf222 were both highly expressed in S4, S5-stage buds, calyx, and the stamen. RNA-seq identified differentially expressed mRNAs and lncRNAs (long non-coding RNAs) that were significantly enriched in pollen wall assembly, pollen development, and pollen coat. Our findings suggest that an energy supply disorder caused by orf222/orf138/orf261b may inhibit a series of nuclear pollen development-related genes. Our study shows that BVRC-CMS96 is a valuable CMS system, and our detailed molecular analysis will facilitate its application in Chinese cabbage breeding.

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