Genome-Wide Analysis of Sugar Transporters Identifies the gtsA Gene for Glucose Transportation in Pseudomonas stutzeri A1501

文献类型： 外文期刊

第一作者： Liu, Yaqun

作者： Liu, Yaqun;Liu, Zhu;Liu, Yaqun;Shang, Liguo;Zhan, Yuhua;Lin, Min;Yan, Yongliang

作者机构：

关键词： Pseudomonas stutzeri A1501; sugar-transport system; glucose; gtsA gene; biological nitrogen fixation

期刊名称：MICROORGANISMS （ 影响因子：4.128； ）

ISSN：

年卷期： 2020 年 8 卷 4 期

页码：

收录情况： SCI

摘要： Pseudomonas stutzeri A1501 possesses an extraordinary number of transporters which confer this rhizosphere bacterium with the sophisticated ability to metabolize various carbon sources. However, sugars are not a preferred carbon source for P. stutzeri A1501. The P. stutzeri A1501 genome has been sequenced, allowing for the homology-based in silico identification of genes potentially encoding sugar-transport systems by using established microbial sugar transporters as a template sequence. Genomic analysis revealed that there were 10 sugar transporters in P. stutzeri A1501, most of which belong to the ATP-binding cassette (ABC) family (5/10); the others belong to the phosphotransferase system (PTS), major intrinsic protein (MIP) family, major facilitator superfamily (MFS) and the sodium solute superfamily (SSS). These systems might serve for the import of glucose, galactose, fructose and other types of sugar. Growth analysis showed that the only effective medium was glucose and its corresponding metabolic system was relatively complete. Notably, the loci of glucose metabolism regulatory systems HexR, GltR/GtrS, and GntR were adjacent to the transporters ABC(MalEFGK), ABC(GtsABCD), and ABC(MtlEFGK), respectively. Only the ABC(GtsABCD) expression was significantly upregulated under both glucose-sufficient and -limited conditions. The predicted structure and mutant phenotype data of the key protein GtsA provided biochemical evidence that P. stutzeri A1501 predominantly utilized the ABC(GtsABCD) transporter for glucose uptake. We speculate that gene absence and gene diversity in P. stutzeri A1501 was caused by sugar-deficient environmental factors and hope that this report can provide guidance for further analysis of similar bacterial lifestyles.

分类号：

  • 相关文献
作者其他论文 更多>>
  • Structural insights into the mechanism of phosphate recognition and transport by XPR1

    作者：Zhang, Wenhui;Chen, Yanke;Guan, Zeyuan;Tang, Meng;Du, Zhangmeng;Zhang, Jie;Cheng, Meng;Zuo, Jiaqi;Liu, Yan;Wang, Qiang;Liu, Yanjun;Zhang, Delin;Yin, Ping;Ma, Ling;Liu, Zhu;Wang, Yong;Liu, Zhu

    关键词：

  • Isotope Ratio Mass Spectrometry (IRMS)-Based Authentication of the Geographic Origins of Volvariella volvacea (Bull.) Singer

    作者：Liu, Xing;Liu, Zhu;Li, Peijun;Liu, Xing;Rao, Qinxiong;Zhang, Qicai;Geng, Hao;Lu, Yangyang;Chen, Shanshan;Song, Weiguo;Liu, Xing;Rao, Qinxiong;Zhang, Qicai;Geng, Hao;Lu, Yangyang;Chen, Shanshan;Song, Weiguo;Liu, Zhu;Li, Peijun

    关键词：Volvariella volvacea; stable isotope; nutrition component; geographic origin; PLS-DA

  • EasyMetagenome: A user-friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research

    作者：Bai, Defeng;Xun, Jiani;Ma, Chuang;Luo, Hao;Yang, Haifei;Hou, Huiyu;Lv, Hujie;Wan, Xiulin;Wang, Yao;Yousuf, Salsabeel;Zeng, Meiyin;Zhang, Tianyuan;Gao, Yunyun;Liu, Yong-Xin;Chen, Tong;Ma, Chuang;Yang, Haifei;Cao, Chen;Cao, Xiaofeng;Cui, Jianzhou;Deng, Yuan-Ping;Deng, Zhaochao;Yu, Hao;Zhang, Chunfang;Dong, Wenxin;Dong, Wenxue;Du, Juan;Fang, Qunkai;Fang, Wei;Fang, Yue;Luan, Yaning;Fu, Fangtian;Fu, Min;Fu, Yi-Tian;Gao, He;Ge, Jingping;Guo, Yuhao;Gong, Qinglong;Lou, Wenbo;Gu, Lunda;Yang, Li;Guo, Peng;Hai, Tang;Liu, Hao;He, Jieqiang;He, Zi-Yang;Huang, Can;Ji, Shuai;Jiang, ChangHai;Jiang, Gui-Lai;Jiang, Lingjuan;Jin, Ling N.;Li, Changchao;Kan, Yuhe;Kang, Da;Kou, Jin;Lam, Ka-Lung;Li, Chong;Li, Fuyi;Li, Liwei;Li, Miao;Li, Xin;Li, Ye;Li, Zheng-Tao;Zhu, Chengshuai;Liang, Jing;Mo, Jiayuan;Lin, Yongxin;Liu, Changzhen;Liu, Danni;Zhang, Jing;Chen, Shifu;Liu, Fengqin;Liu, Jia;Liu, Tianrui;Liu, Tingting;Wang, Xinlong;Liu, Xinyuan;Luo, Yuanyuan;Liu, Yaqun;Liu, Bangyan;Liu, Minghao;Lv, Hujie;Ma, Tengfei;Mai, Zongjiong;Niu, Dongze;Pan, Zhuo;Qi, Heyuan;Shi, Zhanyao;Song, Chunjiao;Sun, Fuxiang;Sun, Yan;Tian, Sihui;Wang, Guoliang;Wang, Hongyang;Wang, Hongyu;Wang, Huanhuan;Wang, Jing;Wang, Jun;Wang, Kang;Wang, Leli;Yao, Xiaofang;Wang, Shao-kun;Xiao, Zufei;Xing, Huichun;Xu, Yifan;Yang, Song;Yan, Shu-yan;Zhang, Yi-Bo;Yang, Yuanming;Lei, Yu;Yuan, Zhengrong;Zhang, Chunge;Zhang, Huimin;Zhang, Na;Zhang, Yupeng;Zhang, Zheng;Zhou, Mingda;Zhou, Yuanping;Zhu, Zhihao;Zhu, Lin;Zhu, Yue;Zou, Hongqin;Zuo, Anna;Dong, Wenxuan;Wen, Tao;Chen, Shifu;Chen, Shifu;Li, Guoliang

    关键词：metagenome; microbiome; microbiota; pipeline; visualization

  • Mining genetic loci and candidate genes related to salt tolerance traits in soybean

    作者：Tian, Rui;Han, Dai;Shi, Xiaolei;Shan, Qimike;Ding, Sunlei;Wu, Yucheng;Yan, Yongliang;Tian, Rui;Shi, Xiaolei;Shan, Qimike;Ding, Sunlei;Yan, Yongliang;Han, Dai;Wu, Yucheng;Zhang, Jinbo;Zhang, Jinbo

    关键词：Soybean; Salt tolerance-related traits; GWAS; Candidate genes

  • From sequences to prebreeding: Chromosome-level genome assemblies, comparative annotation, and a public database for Minghui 86 and Minhui 3301

    作者：Wang, Yue;Chen, Guangwei;Lin, Min;Pan, Leibo;Wang, Feng;Yang, Shaohua;Chen, Zaijie

    关键词：Rice; Minghui 86; Minhui 3301; Genomics; Molecular markers; Database

  • Nitrincola salilacus sp. nov., isolated from Niya Fish Salt Lake Sediment in Xinjiang, Northwest China

    作者：Wang, Qingyu;Xiao, Yi;Gao, Danning;Su, Longyi;Yan, Yongliang;Zhan, Yuhua;Zhou, Zhengfu;Ke, Xiubin

    关键词：chemotaxonomic characterization; comparative genomics; genomic analysis; Nitrincola; salt lake

  • High Resolution Crop Type and Rotation Mapping in Farming-Pastoral Ecotone in China Using Multi-Satellite Imagery and Google Earth Engine

    作者：Hou, Zhenwei;Zang, Huadong;Yang, Yadong;Zeng, Zhaohai;Hou, Zhenwei;Zang, Huadong;Yang, Yadong;Zeng, Zhaohai;Chen, Bangqian;Liu, Yaqun;Manevski, Kiril;Manevski, Kiril;Chen, Fangmiao;Ge, Junyong

    关键词：crop classification; multi-satellite imagery; crop distribution; cropping patterns; farming-pastoral ecotone

微信小程序