A rapid and convenient method for on-site detection of MON863 maize through real-time fluorescence recombinase polymerase amplification

文献类型： 外文期刊

第一作者： Wang, Xiaofu

作者： Wang, Xiaofu;Xie, Shilong;Chen, Xiaoyun;Peng, Cheng;Xu, Xiaoli;Wei, Wei;Xu, Junfeng;Wang, Xiaofu;Chen, Xiaoyun;Peng, Cheng;Xu, Xiaoli;Wei, Wei;Xu, Junfeng;Xie, Shilong;Ma, Tongfu;Cai, Jian

作者机构：

关键词： Genetically modified crop; Recombinase polymerase amplification (RPA); Real-time fluorescence; On-site detection; MON863

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2020 年 324 卷

页码：

收录情况： SCI

摘要： As large-scale planting of genetically modified (GM) crops increases, the development of a rapid and convenient method for on-site detection of GM crops is important. We combined the advantages of recombinase polymerase amplification (RPA) and fluorescence detection to establish a rapid, sensitive, specific, and simple detection platform for on-site detection of MON863 maize. Test samples were added directly to the platform after simple pre-treatment with a DNA extraction-free method. Results were obtained through real-time monitoring with a portable instrument, which facilitated sample-in/answer-out on-site detection. The entire detection process, including sample preparation, RPA and identification of amplification results, was accomplished in approximately 10 min. Furthermore, the detection was achieved with a simple and inexpensive portable device. This method has high potential for application in other fields requiring rapid detection of DNA targets, such as in field research, resource-limited areas, and science education.

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