Cloning, Secretory Expression and Characterization of a Unique pH-Stable and Cold-Adapted Alginate Lyase

文献类型： 外文期刊

第一作者： Wang, Zhi-Peng

作者： Wang, Zhi-Peng;Ji, Xiao-Feng;Wang, Hai-Ying;Wang, Zhi-Peng;Cao, Min;Li, Bing;Zhang, Xin-Yue;Zhang, Yue-Qi;Ji, Xiao-Feng;Wang, Hai-Ying;Wang, Hai-Ying

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关键词： alginate lyase; cold-adapted; pH-stable; NaCl-independent

期刊名称：MARINE DRUGS （ 影响因子：5.118； 五年影响因子：5.951 ）

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年卷期： 2020 年 18 卷 4 期

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收录情况： SCI

摘要： Cold-adapted alginate lyases have unique advantages for alginate oligosaccharide (AOS) preparation and brown seaweed processing. Robust and cold-adapted alginate lyases are urgently needed for industrial applications. In this study, a cold-adapted alginate lyase-producing strain Vibrio sp. W2 was screened. Then, the gene ALYW201 was cloned from Vibrio sp. W2 and expressed in a food-grade host, Yarrowia lipolytica. The secreted Alyw201 showed the activity of 64.2 U/mL, with a molecular weight of approximate 38.0 kDa, and a specific activity of 876.4 U/mg. Alyw201 performed the highest activity at 30 degrees C, and more than 80% activity at 25-40 degrees C. Furthermore, more than 70% of the activity was obtained in a broad pH range of 5.0-10.0. Alyw201 was also NaCl-independent and salt-tolerant. The degraded product was that of the oligosaccharides of DP (Degree of polymerization) 2-6. Due to its robustness and its unique pH-stable property, Alyw201 can be an efficient tool for industrial production.

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