Characterization of hexaconazole-degrading strain Sphingobacterium multivorum and analysis of transcriptome for biodegradation mechanism

文献类型： 外文期刊

第一作者： An, Xiaokang

作者： An, Xiaokang;Tian, Chunyan;Xu, Jun;Dong, Fengshou;Liu, Xingang;Wu, Xiaohu;Zheng, Yongquan

作者机构：

关键词： Hexaconazole; Microbial biodegradation; Sphingobacterium multivorum; Metabolite; Transcriptome

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：7.963； 五年影响因子：7.842 ）

ISSN： 0048-9697

年卷期： 2020 年 722 卷

页码：

收录情况： SCI

摘要： Hexaconazole is a triazole fungicide, which is stable and difficult to degrade in the environment. The existence of hexaconazole could pose a certain risk to the environment and the health of living organisms. In this study, an efficient degradation strain B-3 (Sphingobacterium multivorum) of hexaconazole was isolated from sewage, activated sludge, and soil. The degradation efficiency of hexaconazole can reach 85.6% in 6 days at a temperature of 32.5 degrees C, pH of 6.31, initial inoculum of 0.4 g L-1 and initial concentration of hexaconazole of 50 mg L-1. During degradation, three metabolites (M1: 2-(2, 4-dichlorophenyl)-1-(1H-1, 2, 4-triazol-1-yl) hexane-2, 5-diol; M2: 2-(2, 4-dichlorophenyl) hexane-1, 2-diol; M3: 1H-1, 2, 4-triazole) were identified. Moreover, 45.6% hexaconazole can be degraded in 60 days in natural soil containing B-3. The results of the transcriptomesequencing indicated the presence of 864 differential genes, in which aldehyde dehydrogenase, monooxygenase, RND transporters, and ABC transporters were up-regulated. The generation of 2-(2, 4-dichlorophenyl)-1-(1H-1, 2, 4-triazol-1-yl) hexane-2, 5-diol may be due to the participation of monooxygenase. (C) 2020 Elsevier B.V. All rights reserved.

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