Extraction and Quantification of Sulforaphane and Indole-3-Carbinol from Rapeseed Tissues Using QuEChERS Coupled with UHPLC-MS/MS

文献类型： 外文期刊

第一作者： Yu, Xu

作者： Yu, Xu;Ma, Fei;Zhang, Liangxiao;Li, Peiwu;Yu, Xu;Ma, Fei;Zhang, Liangxiao;Li, Peiwu;Yu, Xu;Ma, Fei;Li, Peiwu;Ma, Fei;Zhang, Liangxiao;Li, Peiwu;Zhang, Liangxiao;Li, Peiwu

作者机构：

关键词： sulforaphane; indole-3-carbinol; QuEChERS; quantification; UHPLC-MS; MS

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN：

年卷期： 2020 年 25 卷 9 期

页码：

收录情况： SCI

摘要： Rapeseed (Brassica napus L.) is rich in phenols, vitamins, carotenoids, and mineral elements, such as selenium. Additionally, it contains the active ingredients sulforaphane and indole-3-carbinol, which have been demonstrated to have pharmacological effects. In this study, sulforaphane and indole-3-carbinol were extracted and quantified from rapeseeds using quick, easy, cheap, effective, rugged and safe (QuEChERS) method coupled with ultra high performance liquid chromarography tandem mass spectrometry (UHPLC-MS/MS). The major parameters for extraction and purification efficiency were optimized, including the hydrolysis reaction, extraction condition and type and amount of purification adsorbents. The limit of detection (LOD) and the limit of quantification (LOQ) for sulforaphane were 0.05 mu g/kg and 0.15 mu g/kg, and for indole-3-carbinol were 5 mu g/kg and 15 mu g/kg, respectively. The developed method was used to successfully analyze fifty rapeseed samples. The QuEChERS coupled with UHPLC-MS/MS simultaneously detect sulforaphane and indole-3-carbinol in vegetable matrix and evaluate the quality and nutrition of rapeseed samples.

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