Molecular cloning and functional characterization of duck DEAD (Asp-Glu-Ala-Asp) box RNA helicase 3 (DDX3X)

文献类型： 外文期刊

第一作者： Zhang, Rongrong

作者： Zhang, Rongrong;Zhu, Xinyu;Liu, Shudan;Xiao, Shaobo;Fang, Liurong;Zhang, Rongrong;Wang, Honglin;Wang, Zui;Lu, Qin;Shao, Huabin

作者机构：

关键词： DDX3X; Duck; Interferon-beta

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）

ISSN： 0006-291X

年卷期： 2020 年 527 卷 2 期

页码：

收录情况： SCI

摘要： DEAD (Asp-Glu-Ala-Asp) box RNA helicase 3 (DDX3X) is demonstrated to have crucial functions in the antiviral immune response. To our knowledge, little information focuses on the function of duck DDX3X. In this study, duck DDX3X (duDDX3X) was cloned and its role in the type I interferon (IFN) signaling pathway was investigated using duck embryo fibroblast (DEF) cells. Full-length duDDX3X cDNA encodes 652 amino acid residues and contains a DEADc domain and a HELICc domain. According to tissue distribution analysis, duDDX3X mRNA was widely expressed in different tissues, especially the spleen and the liver. Overexpression of duDDX3X in DEF cells induced IFN-beta by activating transcription factors IRF1 and NF-kappa B. Knockdown of duDDX3X in DEF cells with siRNA significantly reduced IFN-beta expression induced by poly(I:C), a double-stranded RNA (dsRNA) analog. Our results demonstrated that duck DDX3X was involved in the dsRNA-mediated type I IFN signaling pathway in DEF cells. (C) 2020 Elsevier Inc. All rights reserved.

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