An Orphan Pheromone Receptor Affects the Mating Behavior of Helicoverpa armigera

文献类型： 外文期刊

第一作者： Cao, Song

作者： Cao, Song;Huang, Tianyu;Liu, Yang;Wang, Guirong;Cao, Song;Shen, Jie;Cao, Song;Shen, Jie;Wang, Guirong

作者机构：

关键词： Helicoverpa armigera; pheromone receptor; CRISPR; Cas9; mating behavior; EAG

期刊名称：FRONTIERS IN PHYSIOLOGY （ 影响因子：4.566； 五年影响因子：4.804 ）

ISSN： 1664-042X

年卷期： 2020 年 11 卷

页码：

收录情况： SCI

摘要： The Lepidoptera is the second largest insect order, which has the most extensive knowledge of sex pheromones and mechanisms of pheromone communication since the identification of the first insect pheromone in Bombyx mori. In the past 15 years, pheromone receptors have been identified and functionally characterized in many moth species. HarmOR14 is a typical pheromone receptor of Helicoverpa armigera which showed no response to the tested pheromones in Xenopus oocyte expression system, but its orthologous gene in Heliothis virescens, HvirOR14 could be activated by pheromones in the same expression system. To assess the possible functions of OR14 in vivo, in this study, we knocked out this gene using CRISPR/Cas9 system and compared the mating behaviors and EAG response to pheromones between the wild type and mutant strains. Our results showed that OR14 mutants did not affect the mating rate or the EAG response to pheromones but could prolong the mating duration and change the mating time in undefined manners, which extends our understanding to this kind of pheromone receptors.

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