Investigation and comparison of the protective activities of three functional proteins-lactoferrin, alpha-lactalbumin, and beta-lactoglobulin-in cerebral ischemia reperfusion injury

文献类型： 外文期刊

第一作者： Li, Hui-Ying

作者： Li, Hui-Ying;Li, Peng;Yang, Huai-Gu;Yao, Qian-Qian;Huang, Sheng-Nan;Wang, Jia-Qi;Zheng, Nan;Li, Peng

作者机构：

关键词： lactoferrin; alpha-lactalbumin; beta-lactoglobulin; cerebral ischemia reperfusion; toll-like receptor 4

期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.034； 五年影响因子：4.354 ）

ISSN： 0022-0302

年卷期： 2020 年 103 卷 6 期

页码：

收录情况： SCI

摘要： The objective of this study was to evaluate the protection conferred by lactoferrin, alpha-lactalbumin, and beta-lactoglobulin in cerebral ischemia reperfusion (I/R) injury. Rat pheochromocytoma (PC12) cells were used to construct an oxygen and glucose deprivation model in vitro, and ICR mice underwent carotid artery "ligationrelaxation" to construct a cerebral I/R injury model in vivo. The levels of toll-like receptor 4 (TLR4) and downstream factors including nuclear factor-kappa B, tumor necrosis factor-alpha, and IL-1 beta were measured. Metabonomics detection and data mining were conducted to identify the specific metabolic sponsor of the 3 proteins. The results showed that lactoferrin, alpha-lactalbumin, and beta-lactoglobulin protected neurons from cerebral I/R injury by increasing the level of bopindolol and subsequently inhibiting the TLR4-related pathway to different degrees; beta-lactoglobulin had the strongest activity of the 3 proteins. In summary, this study is the first to investigate and compare the protective effects of lactoferrin, alpha-lactalbumin, and beta-lactoglobulin in a cerebral stroke model. The results implicate TLR4 as a novel target of the 3 bioactive proteins to prevent cerebral I/R injury.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序