Characterization of a specific odorant receptor for linalool in the Chinese citrus fly Bactrocera minax (Diptera: Tephritidae)

文献类型： 外文期刊

第一作者： Liu, Yipeng

作者： Liu, Yipeng;Cui, Zhongyi;Si, Pinfa;Zhou, Qiong;Liu, Yipeng;Liu, Yang;Wang, Guirong;Wang, Guirong;Wang, Guirong

作者机构：

关键词： Bactrocera minax; Transcriptome; Odorant receptor; Xenopus oocytes; Linalool

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.714； 五年影响因子：4.953 ）

ISSN： 0965-1748

年卷期： 2020 年 122 卷

页码：

收录情况： SCI

摘要： Insect sensing of odorants plays important roles in various behaviors, including host location, mate attraction, and oviposition site selection. The odorant receptor (OR) is a key protein in insect environmental odor recognition. Most Diptera studies of ORs have focused on Drosophila and mosquitos, so there little known about ORs in the agricultural pest insects Tephritidae. To understand the olfactory recognition mechanism of Bactrocera minax, we sequenced and analyzed 12 B. minax transcriptomes to identify a total of 59 OR genes. Semi-quantitative reverse transcription PCR (RT-PCR) showed that several BminORs were highly expressed in antennae. Available with a complete open reading frame and expressed in the antennae of both sexes at a higher level than those of other BminORs, BminOR24 was selected for further functional analyses. BminOR24/BminOrco expressed in Xenopus oocytes responded significantly to linalool. The identification of B. minax OR genes lays a foundation for further functional studies of OR genes, and functional characterization of BminOR24 provides insight for improving methods for controlling B. minax, a devastating pest insects.

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