Cry80Aa1, a novel Bacillus thuringiensis toxin with mosquitocidal activity to Culex pipiens pallens
文献类型： 外文期刊
第一作者： Zhou, Yan
作者： Zhou, Yan;Li, Youzhi;Fang, Xuanjun;Wu, Zhongqi;Zhang, Jie;Fang, Xuanjun;Wu, Zhongqi;Fang, Xuanjun;Wu, Zhongqi;Fang, Xuanjun;Wan, Yusong;Jin, Wujun
作者机构：
关键词： Bacillus thuringiensis; Cry80Aa1; Toxin_10; Culex pipiens pallens
期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.841； 五年影响因子：3.368 ）
ISSN： 0022-2011
年卷期： 2020 年 173 卷
页码：
收录情况： SCI
摘要： Bacillus thuringiensis (Bt) can synthesize insecticides to efficiently control insects. In this study, Bt strain S3580-1 with mosquitocidal activity was subjected to whole genome sequencing using an Illumina HiSeq 2000 system. A novel toxin, Cry80Aa1, was identified based on the resulting data. A conserved domain analysis revealed that Cry80Aa1 includes the Ricin_B _lectin domain (located at 10-150) and the Toxin_10 domain (located at 155-353). Phylogenetic tree analysis showed that Cry80Aa1 was in a distinct Glade significantly distinguished from the known Cry proteins containing the Toxin_10 domain. Bioassays demonstrated that the Cry80Aa1 protein exhibited toxicity to third instar larvae of Culex pipiens pallens (LC50: 71.9 mu g/mL; 95% FL: 59.5-122.7 mu g/mL).
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