Degradation of sulphated polysaccharides from Grateloupia livida and antioxidant activity of the degraded components
文献类型: 外文期刊
第一作者: Chen, Shengjun
作者: Chen, Shengjun;Liu, Huan;Yang, Xianqing;Li, Laihao;Qi, Bo;Hu, Xiao;Ma, Haixia;Li, Chunsheng;Pan, Chuang;Chen, Shengjun;Yang, Xianqing;Li, Laihao;Hu, Xiao;Liu, Huan
作者机构:
关键词: Degradation; Polysaccharide; Grateloupia livida; Antioxidant activity
期刊名称:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES ( 影响因子:6.953; 五年影响因子:6.737 )
ISSN: 0141-8130
年卷期: 2020 年 156 卷
页码:
收录情况: SCI
摘要: Degradation of sulphated polysaccharides from Grateloupia livida (GLP) was studied using H2O2-Vc combined with microwave treatment. Optimal conditions were determined after the Box-Behnken response surface analysis. The best degradation conditions were as follows: microwave power 250 W. microwave temperature 54 degrees C, the concentration of H2O2 3 mM, degradation time 33 min. Under these conditions, the DPPH scavenging activity of the degraded GLP (DGLP) was 71.39 +/- 0.17% at a concentration of 6.0 mg/mL. The degradation method is fast and effective, which is beneficial to improve the antioxidant activity of polysaccharides. Two sulphated polysaccharide constituents (DGLP-1 and DGLP-2) were isolated from DGLP and characterized, and their antioxidant activities were determined. The two polysaccharides had high sulphate content (sulphate content: 25.18-25.26%), contained galactose as the major monosaccharide, and had molecular weights of 158.49 +/- 0.43 and 18.06 +/- 0.92 kDa, respectively. Antioxidant assays suggested that DGLP-1 and especially DGLP-2 possessed good antioxidant properties. (C) 2020 Elsevier B.V. All rights reserved.
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