Characterization and antioxidant activities of intracellular polysaccharides from Agaricus bitorquis (QueL.) Sacc. Chaidam ZJU-CDMA-12

文献类型： 外文期刊

第一作者： Kuang, Hui

作者： Kuang, Hui;Wang, Fengju;Chen, Qihe;Jiao, Yingchun;Wang, Wei

作者机构：

关键词： Antioxidant activities; Polysaccharides; Agaricus bitorquis (QueL.) Sacc. Chaidam

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2020 年 156 卷

页码：

收录情况： SCI

摘要： The antioxidant activities of polysaccharides from the fruiting body (PFB), extracellular polysaccharides (EPS) and intracellular polysaccharides (IPS) from Agaricus bitorquis (QueL.)Sacc. Chaidam 2JU-CDMA-12 in vitro were compared. IPS showed stronger antioxidant activities than PFB and EPS in vitro. Further purification and structure analyses indicated that IPS mainly consisted of three fractions (IPS-I, IPS-II and IPS-III). FT-IR and NMR data indicated that IPS was mainly composed of (1 -> 6)-linked alpha-D-glucose. There are significant differences of antioxidant activities among IPS-I, IPS-II and IPS-III fractions in vitro, and IPS-III showed stronger antioxidant activity than IPS-I and IPS-II. IPS-III also possesses a potent antioxidant ability inside HepG2 cells, and it could protect HepG2 cells from H2O2-induced cytotoxicity by scavenging overproduced cellular ROS and inhibiting SOD, CAT and GSH depletion to weaken lipid peroxidation. These findings suggested that IPS-HI could be a novel antioxidant and that it could afford protection against H2O2-induced cytotoxicity and oxidative stress. (C) 2019 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序