Biosynthesis of antioxidative enzymes and polyphenolics content in calli cultures of Prunella vulgaris L. in response to auxins and cytokinins

文献类型： 外文期刊

第一作者： Fazal, Hina

作者： Fazal, Hina;Shah, Jahangir;Khan, Haji;Abbasi, Bilal Haider;Noureen, Bushra;Ahmad, Nisar;Akbar, Fazal;Uddin, Muhammad Nazir;Ali, Mohammad;Ma, Dan;Liu, Chuanliang

作者机构：

关键词： Prunella vulgaris; calli cultures; antioxidative enzymes; polyphenolics; antioxidant activity

期刊名称：ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY （ 影响因子：5.678； 五年影响因子：5.246 ）

ISSN： 2169-1401

年卷期： 2020 年 48 卷 1 期

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收录情况： SCI

摘要： Prunella vulgaris L. is one of the therapeutic herbs containing various polyphenolics, which is used for multiple medicinal purposes. In this study, plant growth regulators (PGRs)-induced calli cultures from seed-derived leaf explants were exploited for the production of stress enzymes and polyphenolics. A growth curve was plotted for each PGR for 49 days period, which showed a distinct lag, log and decline phases. Here, the combination of naphthalene acetic acid (NAA) and 6-benzyleadenine (BA; 0.5 and 2.0 mg l(-1)) produced maximum fresh (6.32 FW-g/100 ml) and dry biomass (0.75 DW-g/100 ml) in contrast to control. The maximum synthesis of SOD (0.0154 FW-nM/min/mg) was detected on media comprising mixture of NAA and BA (1.5 mg l(-1)), while POD enzyme (0.366 FW-nM/min/mg) was higher at 0.5 mg l(-1) NAA and 2, 4-dichlorophenoxy acetic acid. Further, NAA and BA (1.5 and 2.0 mg l(-1)) boosted up the synthesis of phenolics (18.83 GAE-mg/g-DW) and flavonoids content (18.05 RE-mg/g-DW) than control. Moreover, NAA of 1.0 and 2.0 mg l(-1) were found supportive for maximum antioxidant activity (87.4%) and total protein (716 mu g BSAE/mg-DW). This study will contribute in the development of cell culture in fermenter and synthesis of antioxidant secondary metabolites for commercial uses.

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