Water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan enhanced the immunogenicity of inactivated porcine parvovirus vaccine vaccination on sows against porcine parvovirus infection

文献类型： 外文期刊

第一作者： Zhou, Mo

作者： Zhou, Mo;Qu, Wanying;Zhao, Kai;Zhou, Mo;Qu, Wanying;Sun, Yanwei;Zhao, Kai;Liang, Lin;Cui, Shangjin;Jin, Zheng;Liang, Lin;Cui, Shangjin

作者机构：

关键词： Porcine parvovirus; N-2-Hydroxypropyl trimethyl ammonium chloride chitosan; Vaccine adjuvant; Inactivated vaccine

期刊名称：IMMUNOLOGY LETTERS （ 影响因子：3.685； 五年影响因子：3.54 ）

ISSN： 0165-2478

年卷期： 2020 年 223 卷

页码：

收录情况： SCI

摘要： Porcine parvovirus (PPV) is one of the most common and important virus causes of infectious infertility in swine throughout the world. Inactivated PPV vaccine is broadly used, however, there is no appropriate immunomodulatory adjuvant for enhancing present vaccines and developing new ones. Therefore, in this study, the water-soluble N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (N-2-HACC) was synthesized, the adjuvant potential of chitosan derivative was evaluated in inactivated PPV vaccine. Twenty adult healthy sows were assigned to four groups and vaccinated with synthesized PPV/N-2-HACC, commercial inactivated vaccine, N-2-HACC adjuvant and PBS. After insemination, all sows were challenged with the homologous PPV-H strain. In vivo immunization showed that sows immunized with the PPV/N-2-HACC induced more long-lasting HI antibodies and strong immune responses. Importantly, immunization of PPV/N-2-HACC significantly protected sows from homologous PPV-H strain infection. However, immunization of PPV/N-2-HACC didn't change the level of IL-2, IL-4 and IFN-gamma and the production of CD4+, CD8 + T lymphocyte. The results indicated that PPV/N-2-HACC protect PPV infection mainly through enhancing the humoral immunity rather than cellular immunity. In addition, the mummified fetuses were observed from the control groups, but neither of the two vaccine groups. In conclusion, these results suggest that N-2-HACC can be exploited as an effective adjuvant for vaccine development, and the PPV/N-2-HACC are potent immunization candidates against PPV infection.

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