Rapid DNA extraction and microfluidic LAMP system in portable equipment for GM crops detection

文献类型： 外文期刊

第一作者： Xiao, Bin

作者： Xiao, Bin;Zhang, Juan;Wang, Nan;Li, Liang;Pang, Xiangyi;Liu, Chuan;Huang, Fengchun;Chen, Ailiang;Wang, Mengyu;Fu, Wei;Chen, Hong;Wang, Haoqian

作者机构：

关键词： Fast extraction method; Hand-held chip; LAMP; Portable analyzer; Genetically modified crops; On -spot detection

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：8.4； 五年影响因子：7.2 ）

ISSN：

年卷期： 2024 年 411 卷

页码：

收录情况： SCI

摘要： Genetically modified food has come under suspicion because of the potential safety risks. Only a limited number of target genes could be detected by traditional methods. Here, customized-fast DNA extraction methods and a LAMP (Loop-mediated isothermal amplification) system were developed combining with a hand-held chip, a portable analyzer, and an Android App for the qualitative detection of genetically modified crops. DNA of seeds and leaves was obtained within 5 minutes. Primers were designed with high specificity and sensitivity (10 copies) for later LAMP amplification. Crude DNA was added to the chip pre-embedded with customizable primers by quantitative dropper and the whole detection process was less than 40 minutes without any large-scale instrument. The 0.1% quality percentage of GM samples (maize or soybean) could be detected by the detection system. The detection system with its low workload, high accuracy, and stability offers a novel and effective approach for the rapid detection of GM crops and many other food safety areas in the field.

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