Effects of GABA and Vigabatrin on the Germination of Chinese Chestnut Recalcitrant Seeds and Its Implications for Seed Dormancy and Storage

文献类型： 外文期刊

第一作者： Du, Changjian

作者： Du, Changjian;Chen, Wei;Wu, Yanyan;Sun, Jiacheng;Ji, Jing;Shi, Shengqing;Wang, Guangpeng;Zhao, Jiabing;Yan, Donghui;Jiang, Zeping

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关键词： chestnut; GABA; seed germination; carbon metabolism; nitrogen metabolism

期刊名称：PLANTS-BASEL （ 影响因子：3.935； ）

ISSN：

年卷期： 2020 年 9 卷 4 期

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收录情况： SCI

摘要： Recalcitrant chestnut seeds are rich in gamma-aminobutyric acid (GABA), which negatively regulates adventitious root development by altering carbon/nitrogen metabolism. However, little is known regarding the role of this metabolite in chestnut seeds. In this study, we investigated the effects of GABA changes on the germination of chestnut seeds treated with exogenous GABA and vigabatrin (VGB, which inhibits GABA degradation). Both treatments significantly inhibited seed germination and primary root growth and resulted in the considerable accumulation of H2O2, but the endogenous GABA content decreased before germination at 48 h. Soluble sugar levels increased before germination, but subsequently decreased, whereas starch contents were relatively unchanged. Changes to organic acids were observed at 120 h after sowing, including a decrease and increase in citrate and malate levels, respectively. Similarly, soluble protein contents increased at 120 h, but the abundance of most free amino acids decreased at 48 h. Moreover, the total amino acid levels increased only in response to VGB at 0 h. Accordingly, GABA and VGB altered the balance of carbon and nitrogen metabolism, thereby inhibiting chestnut seed germination. These results suggested that changes to GABA levels in chestnut seeds might prevent seed germination. The study data may also help clarify the dormancy and storage of chestnut seeds, as well as other recalcitrant seeds.

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