Purification of cell-derived Japanese encephalitis virus by dual-mode chromatography

文献类型： 外文期刊

第一作者： Zhang, Fuliang

作者： Zhang, Fuliang;Zhang, Yihua;Zhang, Fuliang;Luo, Jun;Teng, Man;Xing, Guangxu;Guo, Junqing

作者机构：

关键词： Capto Core 700; Japanese encephalitis virus; purification; Sepharose 4 fast flow; tangential-flow filtration

期刊名称：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY （ 影响因子：2.431； 五年影响因子：2.124 ）

ISSN： 0885-4513

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收录情况： SCI

摘要： Purification of the enveloped virus poses a challenge as one must retain viral infectivity to preserve immunogenicity. The traditional process of virus purification is time-consuming, laborious and hard to scale up. Here, a rapid, simple and extensible laboratory program for the purification of Japanese encephalitis virus (JEV) was developed by using differential centrifugation, ultrafiltration, Sepharose 4 fast flow gel chromatography, and Capt Core 700 chromatography. The entire process recovered 61.64% of the original virus, and the purified virus particles maintained good activity and immunogenicity. The purification process described has potential application in large-scale production of high-purity JEV.

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