Localization shift of a sugar transporter contributes to phloem unloading in sweet watermelons

文献类型： 外文期刊

第一作者： Ren, Yi

作者： Ren, Yi;Sun, Honghe;Zong, Mei;Guo, Shaogui;Li, Maoying;Zhang, Jie;Tian, Shouwei;Wang, Jinfang;Yu, Yongtao;Gong, Guoyi;Zhang, Haiying;He, Hongju;Liu, Fan;Xu, Yong;Ren, Zhijie;Li, Legong;Zhao, Jianyu;Zhang, Xiaolan;Fei, Zhangjun;Fei, Zhangjun

作者机构：

关键词： localization shift; sugar content; sugar phloem unloading; sugar transporter; watermelon

期刊名称：NEW PHYTOLOGIST （ 影响因子：10.151； 五年影响因子：10.475 ）

ISSN： 0028-646X

年卷期： 2020 年 227 卷 6 期

页码：

收录情况： SCI

摘要： Unloading sugar from sink phloem by transporters is complex and much remains to be understood about this phenomenon in the watermelon fruit. Here, we report a novelvacuolar sugar transporter(ClVST1) identified through map-based cloning and association study, whose expression in fruit phloem is associated with accumulation of sucrose (Suc) in watermelon fruit.ClVST1(97)knockout lines show decreased sugar content and total biomass, whereas overexpression ofClVST1(97)increases Suc content. Population genomic and subcellular localization analyses strongly suggest a single-base change at the coding region ofClVST1(97)as a major molecular event during watermelon domestication, which results in the truncation of 45 amino acids and shifts the localization of ClVST1(97)to plasma membranes in sweet watermelons. Molecular, biochemical and phenotypic analyses indicate thatClVST1(97)is a novel sugar transporter for Suc and glucose efflux and unloading. Functional characterization ofClVST1provides a novel strategy to increase sugar sink potency during watermelon domestication.

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