Metabolic responses in Scophthalmus maximus kidney subjected to thermal stress

文献类型： 外文期刊

第一作者： Yang, Shuangshuang

作者： Yang, Shuangshuang;Zhao, Tingting;Ma, Aijun;Huang, Zhihui;Liu, Zhifeng;Cui, Wenxiao;Zhang, Jinsheng;Zhu, Chunyue;Guo, Xiaoli;Yuan, Chenhao;Yang, Shuangshuang;Zhao, Tingting;Ma, Aijun;Huang, Zhihui;Liu, Zhifeng;Cui, Wenxiao;Zhang, Jinsheng;Zhu, Chunyue;Guo, Xiaoli;Yuan, Chenhao;Zhao, Tingting;Cui, Wenxiao;Zhang, Jinsheng;Zhu, Chunyue;Guo, Xiaoli

作者机构：

关键词： Scophthalmus maximus; Thermal stress; Metabolomics; Amino acid metabolism; Carbohydrate metabolism

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2020 年 103 卷

页码：

收录情况： SCI

摘要： Turbot (Scophthalmus maximus) is an economically important marine fish cultured in China. In this study, fish in the experimental group were exposed to four temperatures: 15, 20, 25 and 28 degrees C. Metabolomics analysis and quantitative real-time PCR were used to assess changes in metabolic profiling and gene expression associated with thermal stress. The results showed the levels of heat shock protein 70 (HSP70), heat shock protein 90 (HSP90), blood creatinine and cortisol in S. maximus were all significantly upregulated (P < 0.05), indicating a stress response at 25 degrees C or higher. Challenge with thermal stress significantly increased expression levels of succinate dehydrogenase (SDH), fructose-1, 6-bisphosphatase (FBPase), malate dehydrogenase (MDH), cytosolic phosphoenolpyruvate carboxykinase (cPEPCK), glucose-6-phosphatase (G6Pase) and aspartate aminotransferase (AST) (P < 0.05). However, there was no effect on the expression levels of lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and mitochondrial phosphoenolpyruvate carboxykinase (mPEPCK). Moreover, high temperature decreased levels of glycogenic amino acids, including histidine, threonine, glutamine, phenylalanine, arginine, serine, tyrosine, methionine and isoleucine. These findings suggest a significant correlation between gene expression and regulation of carbohydrate and amino acid metabolism in heat-stressed S. maximus kidney. In addition, the maintenance of aerobic metabolism and activation of gluconeogenesis appeared to be a critical metabolic strategy in combating heat stress in turbot kidney.

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