Identification of a chitosanase from the marine metagenome and its molecular improvement based on evolution data

文献类型： 外文期刊

第一作者： Han, Yanshuo

作者： Han, Yanshuo;Sun, Jilu;Tian, Jian;Han, Yanshuo;Guan, Feifei;Wu, Ningfeng;Tian, Jian

作者机构：

关键词： Marine metagenome; Chitooligosaccharides; Chitosanase; Multipoint mutation; Thermal stability

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2020 年 104 卷 15 期

页码：

收录情况： SCI

摘要： Chitooligosaccharides have important application value in the fields of food and agriculture. Chitosanase can degrade chitosan to obtain chitooligosaccharides. The marine metagenome contains many genes related to the degradation of chitosan. However, it is difficult to mine valuable genes from large gene resources. This study proposes a method to screen chitosanases directly from the marine metagenome. Chitosanase genechis1754was identified from the metagenome and heterologously expressed inEscherichia coli. The optimal temperature and pH of CHIS1754 were 55 degrees C and 5.5, respectively. A mutant, CHIS1754T, with 15 single point mutations designed based on molecular evolution data was also expressed inE. coli. The results indicated that the thermal stability of CHIS1754T was significantly improved, as theT(m)showed an increase of similar to 7.63 degrees C. Additionally, thek(cat)/K(m)of CHIS1754T was 4.8-fold higher than that of the wild type. This research provides new theories and foundations for the excavation, modification, and industrial application of chitosanases.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序