In vitro induction and characterization of hexaploidPennisetum x advena, an ornamental grass

文献类型： 外文期刊

第一作者： Yue, Yuesen

作者： Yue, Yuesen;Fan, Xifeng;Hu, Yaofang;Han, Chao;Li, Hui;Teng, Wenjun;Zhang, Hui;Teng, Ke;Wen, Haifeng;Yang, Xuejun;Wu, Juying

作者机构：

关键词： Pennisetum x advena; Polyploidization; Flow cytometry; Triploid; Hexaploid

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN： 0167-6857

年卷期： 2020 年 142 卷 2 期

页码：

收录情况： SCI

摘要： Key message Nine independent hexaploids were generated from calli derived from immature inflorescence tissue of triploidPennisetum x advenaby a colchicine treatment, and their morphological and anatomical traits were evaluated. Pennisetum x advena(purple fountain grass) is an ornamental grass with considerable commercial value. In vitro induction of hexaploids and evaluation of the morphological and anatomical traits inP. xadvenawere conducted in this study. Nine independent hexaploids were generated from calli, which was initiated from immature inflorescence tissue of triploidP. xadvena, treated with 0.1% colchicine for 24 h. Their ploidy levels were first screened by flow cytometry and further identified by chromosome counting. Compared with the triploids, the hexaploids showed a significant delay in flowering, and reduction in plant height, the number of tillers and number of inflorescences per plant, leaf length and width, inflorescence diameter, and the number of florets per inflorescence, but an increase in floret fresh weight, and anther diameter and length. Pollen grains in the hexaploids were well developed, and their fertility was restored. The production of hexaploids ofP. xadvenaby colchicine treatment, and assessment of their growth parameters and pollen fertility, provide important information for future breeding ofP. xadvena.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序