Transcriptional profiling of underground interaction of two contrasting sunflower cultivars with the root parasitic weedOrobanche cumana

文献类型： 外文期刊

第一作者： Yang, Chong

作者： Yang, Chong;Yang, Chong;Fu, Fei;Zhang, Na;Wang, Jiansu;Hu, Luyang;Islam, Faisal;Zhou, Weijun;Yang, Chong;Fu, Fei;Zhang, Na;Wang, Jiansu;Hu, Luyang;Islam, Faisal;Zhou, Weijun;Bai, Quanjiang;Yun, Xiaopeng

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关键词： Sunflower root; Host defense; Orobanche cumana; Pathogenesis; Candidate effector

期刊名称：PLANT AND SOIL （ 影响因子：4.192； 五年影响因子：4.712 ）

ISSN： 0032-079X

年卷期： 2020 年 450 卷 1-2 期

页码：

收录情况： SCI

摘要： Aims Orobanche cumanais an obligate root parasite which specifically infects roots ofHelianthus annuusL., causing severe yield and economic losses in agricultural fields worldwide. For an efficient control strategy, it is necessary to understand specialized host defense mechanisms and the pathogenesis ofO. cumanaduring underground interaction with the host. Methods RNA-Seq was used to simultaneously assess the transcriptome of each of two contrasting sunflower cultivars withO. cumanaduring their post-attachment interactions. Secretome prediction was applied to identifyO. cumanaproteins from its transcriptome. Results Infection ofO. cumanatriggered insufficient defense responses in the susceptible cultivar as compared to the resistant one, probably due to the failure to fully recognize parasite effectors. Secretome prediction from theO. cumanatranscriptome enabled robust identification of 180 proteins associated withO. cumanapenetration and infection. Functional annotation of these proteins revealed that they were associated with cell wall degradation, nutrient acquisition and pathogenesis. Additionally, a candidate effector containing a PAR1 domain fromO. cumanawas preliminarily identified as a potential suppressor of plant defense responses. Conclusions Secretome prediction shed lights on the molecular mechanisms thatO. cumanadeployed to manipulate host defense. Successful infection ofO. cumanainhibited host defense responses and turned host roots from a sink to a source for efficient nutrient flux towards the parasite. In summary, these findings provide insight into the mechanism underlyingO. cumanainfection and are important in understanding parasitic-host plant interaction.

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