Mechanism of exogenous cytokinins inducing bulbil formation inLilium lancifoliumin vitro

文献类型： 外文期刊

第一作者： He, Guoren

作者： He, Guoren;Yang, Panpan;Tang, Yuchao;Cao, Yuwei;Qi, Xianyu;Xu, Leifeng;Ming, Jun

作者机构：

关键词： Lilium lancifolium; Cytokinin; Bulbil formation; Bulbil initiation

期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

ISSN： 0721-7714

年卷期： 2020 年 39 卷 7 期

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收录情况： SCI

摘要： Key message The cytokinin pathway promotes the initiation of bulbil formation, and iPA may an important type of cytokinin during bulbil formation inLilium lancifolium. Bulbils are important vegetative reproductive organs in triploidLilium lancifolium. We previously showed that cytokinins are involved in bulbil formation, but how cytokinins participate in bulbil formation is not clear. In this study, bulbil formation was divided into three stages on the basis of anatomical and histological observations: the bulbil initiation stage, bulbil primordium-formation stage and bulbil structure-formation stage. The results indicated that iPA was the most critical cytokinin during the bulbil initiation. qRT-PCR revealed that increased iPA content during bulbil initiation was mainly due to increased expression of cytokinin synthesis genes (IPT1/5) and cytokinin activation genes (LOG1/3/5/7) and significantly decreased expression of the cytokinin degradation geneCKX4. Exogenous 6-BA and lovastatin affected the cytokinin pathway and promoted or inhibited bulbil initiation by increasing or decreasing the content of endogenous iPA, respectively. In summary, we demonstrate that cytokinins positively regulate bulbil formation and provide preliminary insight into the regulatory mechanisms by which the cytokinin pathway promotes bulbil initiation.

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