Nascent Polypeptide-Associated Complex Involved in the Development and Pathogenesis of Fusarium graminearum on Wheat

文献类型： 外文期刊

第一作者： Wang, Xuli

作者： Wang, Xuli;Xie, Xin;Liu, Jin;Wang, Guo-Liang;Qiu, Dewen;Xie, Xin;Wang, Guo-Liang

作者机构：

关键词： Fusarium head blight; Nascent polypeptide-associated complex; Gene knockout; Pathogenicity; Subcellular localization

期刊名称：ENGINEERING （ 影响因子：7.553； 五年影响因子：8.977 ）

ISSN： 2095-8099

年卷期： 2020 年 6 卷 5 期

页码：

收录情况： SCI

摘要： Reliable knowledge on pathogenic agents contributes to effective plant protection. For most plant pathogens, maintaining protein homeostasis (proteostasis) is essential for unfolding the cellular functions to survive and thrive. However, the fungal proteins involved in proteostasis remain poorly characterized in the process of pathogenesis. In this study, we characterized the function of the nascent polypeptide-associated complex (NAC) in Fusarium graminearum (F. graminearum) (FgNAC), one of the top 10 fungal pathogens with predominant scientific/economic importance. We found that FgNAC alpha, a subunit of FgNAC, manifests high structural and functional similarity to its homologous counterparts in yeast and other species. The mutants of F. graminearum lacking NAC alpha are viable but suffer significant defects in vegetative growth, conidial production, and pathogenesis. In addition, we show here that FgNAC alpha can interact with another subunit of NAC (FgNAC beta) in a yeast-two-hybrid assay. The subcellular localization results show that FgNAC alpha and FgNAC beta are predominantly localized in the cytoplasm. Future studies should focus on deciphering the mechanism by which NAC orchestrates protein biogenesis and consequentially modulates development and pathogenesis. (C) 2020 THE AUTHORS. Published by Elsevier LTD on behalf of Chinese Academy of Engineering and Higher Education Press Limited Company.

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