Insight of low-abundance proteins in rice leaves under Cd stress using combinatorial peptide ligand library technology

文献类型： 外文期刊

第一作者： Lin, Xiaoyan

作者： Lin, Xiaoyan;Chai, Shuangshuang;Huang, Siqi;Mou, Renxiang;Cao, Zhaoyun;Cao, Zhenzhen;Chen, Mingxue;Lin, Xiaoyan;Chai, Shuangshuang;Huang, Siqi;Mou, Renxiang;Cao, Zhaoyun;Cao, Zhenzhen;Chen, Mingxue

作者机构：

关键词： Low-abundance proteins; Combinatorial peptide ligand library; Proteomics; Rice leaves; Cd stress

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.142； 五年影响因子：3.863 ）

ISSN： 1618-2642

年卷期： 2020 年 412 卷 22 期

页码：

收录情况： SCI

摘要： Low-abundance proteins (LAPs) play a very important role in interaction, regulation, and metabolism of plant biological processes. A combinatorial peptide ligand library (CPLL) can solve the problem of high-abundance proteins (HAPs) masking LAPs and enlarging the dynamic range of protein concentrations perfectly and be considered as one of the most advanced approaches for plant proteomics research. In this paper, a proper CPLL method to rice leaf proteins was established for the first time and 1056 proteins were identified in rice leaf extracts, and 624 (59.1%) LAPs were newly detected after CPLL. Based on this technology, we detected the response of rice to Cd stress and analyzed the differential LAPs and the biological significance of misexpressed proteins before and after Cd stress by bioinformatics analysis. An important contribution has also been made to a better understanding of the complex mechanisms by which rice adapts to Cd stress.

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