Two mating-type genesMAT1-1-1andMAT1-1-2with significant functions in conidiation, stress response, sexual development, and pathogenicity of rice false smut fungusVillosiclava virens

文献类型： 外文期刊

第一作者： Yong, Mingli

作者： Yong, Mingli;Yu, Junjie;Pan, Xiayan;Yu, Mina;Cao, Huijuan;Song, Tianqiao;Qi, Zhongqiang;Du, Yan;Zhang, Rongsheng;Yin, Xiaole;Liu, Yongfeng;Liu, Wende

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关键词： Villosiclava virens; Mating-type gene; Conidiation; Stress response; Sexual development; Pathogenicity

期刊名称：CURRENT GENETICS （ 影响因子：3.886； 五年影响因子：3.697 ）

ISSN： 0172-8083

年卷期： 2020 年 66 卷 5 期

页码：

收录情况： SCI

摘要： Rice false smut caused byVillosiclava virensis one of the destructive diseases on panicles of rice. Sexual development ofV. virens, controlled by mating-type locus, plays an important role in the prevalence of rice false smut and genetic diversity of the pathogen. However, how the mating-type genes mediate sexual development of theV. virensremains largely unknown. In this study, we characterized the two mating-type genes,MAT1-1-1andMAT1-1-2, inV. virens.MAT1-1-1knockout mutant showed defects in hyphal growth, conidia morphogenesis, sexual development, and increase in the tolerance to salt and osmotic stress. Targeted deletion ofMAT1-1-2not only impaired the sclerotia formation and pathogenicity ofV. virens, but also reduced the production of conidia. TheMAT1-1-2mutant showed increases in tolerance to salt and hydrogen peroxide stress, but decreases in tolerance to osmotic stress. Yeast two-hybrid assay showed that MAT1-1-1 interacted with MAT1-1-2, indicating that those proteins might form a complex to regulate sexual development. In addition, MAT1-1-1 localized in the nucleus, and MAT1-1-2 localized in the cytoplasm. Collectively, our results demonstrate thatMAT1-1-1andMAT1-1-2play important roles in the conidiation, stress response, sexual development, and pathogenicity ofV. virens, thus providing new insights into the function of mating-type gene.

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