Comparative Transcriptomic Analysis to Identify the Genes Related to Delayed Gland Morphogenesis inGossypium bickii

文献类型： 外文期刊

第一作者： Ali, Mushtaque

作者： Ali, Mushtaque;Cheng, Hailiang;Soomro, Mahtab;Li Shuyan;Tufail, Muhammad Bilal;Nazir, Mian Faisal;Feng, Xiaoxu;Zhang, Youping;Zuo Dongyun;Lv Limin;Wang, Qiaolian;Song, Guoli

作者机构：

关键词： Gossypium bickii; RNA-seq; DEGs; WGCNA

期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）

ISSN：

年卷期： 2020 年 11 卷 5 期

页码：

收录情况： SCI

摘要： Cotton is one of the major industrial crops that supply natural fibers and oil for industries. This study was conducted to understand the mechanism of delayed gland morphogenesis in seeds ofGossypium bickii. In this study, we compared glandless seeds ofG. bickiiwith glanded seeds ofGossypium arboreum. High-throughput sequencing technology was used to explore and classify the expression patterns of gland-related genes in seeds and seedlings of cotton plants. Approximately 131.33 Gigabases of raw data from 12 RNA sequencing samples with three biological replicates were generated. A total of 7196 differentially-expressed genes (DEGs) were identified in all transcriptome data. Among them, 3396 genes were found up-regulated and 3480 genes were down-regulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations were performed to identify different functions between genes unique to glandless imbibed seeds and glanded seedlings. Co-expression network analysis revealed four modules that were identified as highly associated with the development of glandless seeds. Here the hub genes in each module were identified by weighted gene co-expression network analysis (WGCNA). In total, we have selected 13 genes involved in transcription factors, protein and MYB-related functions, that were differentially expressed in transcriptomic data and validated by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). These selected genes may play an important role for delayed gland morphogenesis. Our study provides comprehensive insight into the key genes related to glandless traits of seeds and plants, and can be further exploited by functional and molecular studies.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序