The abscisic acid receptor geneVvPYL4positively regulates grapevine resistance toPlasmopara viticola

文献类型： 外文期刊

第一作者： Liu, Li

作者： Liu, Li;Huang, Yu-Fei;Liu, Li;Liu, Chang-Yuan;Wang, Hui;Yu, Shu-Yi;Guan, Tian-Shu;Li, Rui-Chun

作者机构：

关键词： Plasmopara viticola; Abscisic acid receptorPYL4; ABA signaling pathway; Virus-induced gene silencing; Defense mechanisms

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN： 0167-6857

年卷期： 2020 年 142 卷 3 期

页码：

收录情况： SCI

摘要： Downy mildew is one of the most serious diseases threatening grapevine cultivation. The PYR/PYL/RCAR proteins, which act as abscisic acid (ABA) receptors, are multifunctional, highly conserved, and reportedly play a crucial role in plant stress resistance in the ABA signaling pathway. In this study, we cloned a putative abscisic acid receptor gene,VvPYL4, from the 'Beta' grapevine cultivar. An analysis of the subcellular localization demonstrated that overexpressed VvPYL4 was localized in both the cytoplasm and the nucleus. The resistance of grapevine cultivars to downy mildew increased after the application of the exogenous hormones MeJA and ABA. The expression ofVvPYL4was significantly induced when exposed toPlasmopara viticola, MeJA, and ABA. The transcript of theVvPYL4gene in 'Beta' grapevine leaves treated with tobacco rattle virus-induced gene silencing (VIGS) was significantly down-regulated. SilencingVvPYL4had a slight effect onP. viticolaresistance. Furthermore, silencingVvPYL4significantly altered the transcripts of ABA and JA responsive genes, including the ABA signaling pathway-related genesABF1, SnRK2.2,and PP2C2,as well as the genes related to the JA signaling pathway,MYC2,JAR1, andJAZ. Altogether, the data provide new evidence thatVvPYL4positively regulates grapevine response to downy mildew. This study also discusses the regulation mechanism ofVvPYL4on ABA and JA signaling transduction. Finally, these results provide a basis for additional research on the mechanisms behind grapevine resistance to downy mildew. Key message SilencingVvPYL4impaired resistance toP. viticola, and this was associated with inhibited the expression of the ABA and JA signaling pathway-related genes.P. viticola, MeJA, and ABA induced the up-regulated expression ofVvPYL4.

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