Rice dwarf and low tillering 10 (OsDLT10) regulates tiller number by monitoring auxin homeostasis

文献类型： 外文期刊

第一作者： Wen, Xiaoxia

作者： Wen, Xiaoxia;Sun, Lianping;Chen, Yuyu;Xue, Pao;Yang, Qinqin;Wang, Beifang;Yu, Ning;Cao, Yongrun;Zhang, Yue;Gong, Ke;Wu, Weixun;Chen, Daibo;Cao, Liyong;Cheng, Shihua;Zhang, Yingxin;Zhan, Xiaodeng;Wen, Xiaoxia;Sun, Lianping;Chen, Yuyu;Xue, Pao;Yang, Qinqin;Wang, Beifang;Yu, Ning;Cao, Yongrun;Zhang, Yue;Gong, Ke;Wu, Weixun;Chen, Daibo;Cao, Liyong;Cheng, Shihua;Zhang, Yingxin;Zhan, Xiaodeng;Wen, Xiaoxia;Sun, Lianping;Chen, Yuyu;Xue, Pao;Yang, Qinqin;Wang, Beifang;Yu, Ning;Cao, Yongrun;Zhang, Yue;Gong, Ke;Wu, Weixun;Chen, Daibo;Cao, Liyong;Cheng, Shihua;Zhang, Yingxin;Zhan, Xiaodeng

作者机构：

关键词： Rice; Tillering; OsDLT10; Axillary bud; Auxin

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN： 0168-9452

年卷期： 2020 年 297 卷

页码：

收录情况： SCI

摘要： Tiller number is a crucial agronomic trait that directly affects the number of effective panicles and yield formation in rice. Here, we report a semi-dwarf and low tillering mutant Osdlt10 (dwarf and low tillering 10) that exhibited reduced filler number, semi-dwarfism, increased grain width, low seed-setting rate, curled leaf tip and a series of abnormalities of agronomic traits. Phenotypic observations showed that Osdlt10 mutants had defects in filler bud formation and grew slowly at the tillering stage. Map-based cloning revealed that LOC_Os10g41310 was the responsible gene for OsDLT10, which was subsequently demonstrated using the CRISPR/Cas9 system and a complementary experiment. Expression pattern analysis indicated that OsDLT10 was primarily expressed in the stem node, the basic part of axillary bud and leaf sheath, pulvinus. The hormone treatment investigation indicated that extremely high of exogenous auxin concentrations can inhibit the expression of OsDLT10. Endogenous auxin content decreased significantly at the base of stem node and axillary bud in Osdlt10 mutants. The results showed that OsDLT10 was related to auxin. qPCR analysis results further showed that the expression levels of auxin transport genes (PINs) and early response genes (IAAs) were significantly increased. The expression levels of WUS-like and FON1 were substantially decreased in the Osdlt10 mutants. These results revealed that OsDLT10 played a critical role in influencing filler number, likely in association with hormone signals and the WUS-CLV pathway, to regulate axillary bud development in rice.

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